In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa

Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam...

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Main Authors:	Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf

id	ukm-13628
recordtype	eprints
spelling	ukm-136282019-11-10T03:42:29Z http://journalarticle.ukm.my/13628/ In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam alone and in various combinations were determined by E-test for 38 imipenem-resistant P. aeruginosa isolates obtained from a Thai hospital. Of the 38 IRPA isolates, 9 (24%) were low-level IRPA (defined as MICs of imipenem 8-32 μg/mL) and 29 (76%) were high-level IRPA (defined as MICs of imipenem >32 μg/mL). The high-level IRPA isolates were susceptible to colistin (90%), piperacillin/tazobactam (72%), and amikacin (52%). The low-level IRPA isolates were susceptible to colistin (100%) and all other antimicrobials tested (78%-89%). The MIC50 value of colistin against both the high-level and low-level IRPA isolates was 1.5 μg/mL. Of all the antimicrobial combinations tested, ceftazidime plus ciprofloxacin displayed the highest percentages of synergistic effects against IRPA isolates (26%, 10/38 isolates) and a high percentages of synergistic effects against high-level IRPA isolates (21, 6/29 isolates), with no antagonistic effects detected. Colistin had the greatest activity against most IRPA isolates among all of the antimicrobials tested, while ceftazidime plus ciprofloxacin showed promise in treating infections caused by IRPA isolates including high-level IRPAs. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf Wanutsanun Tunyapanit, and Pornpimol Pruekprasert, and Kamolwish Laoprasopwattana, and Sureerat Chelae, (2019) In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa. Sains Malaysiana, 48 (5). pp. 1065-1073. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam alone and in various combinations were determined by E-test for 38 imipenem-resistant P. aeruginosa isolates obtained from a Thai hospital. Of the 38 IRPA isolates, 9 (24%) were low-level IRPA (defined as MICs of imipenem 8-32 μg/mL) and 29 (76%) were high-level IRPA (defined as MICs of imipenem >32 μg/mL). The high-level IRPA isolates were susceptible to colistin (90%), piperacillin/tazobactam (72%), and amikacin (52%). The low-level IRPA isolates were susceptible to colistin (100%) and all other antimicrobials tested (78%-89%). The MIC50 value of colistin against both the high-level and low-level IRPA isolates was 1.5 μg/mL. Of all the antimicrobial combinations tested, ceftazidime plus ciprofloxacin displayed the highest percentages of synergistic effects against IRPA isolates (26%, 10/38 isolates) and a high percentages of synergistic effects against high-level IRPA isolates (21, 6/29 isolates), with no antagonistic effects detected. Colistin had the greatest activity against most IRPA isolates among all of the antimicrobials tested, while ceftazidime plus ciprofloxacin showed promise in treating infections caused by IRPA isolates including high-level IRPAs.
format	Article
author	Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae,
spellingShingle	Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
author_facet	Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae,
author_sort	Wanutsanun Tunyapanit,
title	In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
title_short	In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
title_full	In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
title_fullStr	In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
title_full_unstemmed	In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
title_sort	in vitro effects of various antimicrobials alone and in combinations against imipenem-resistant pseudomonas aeruginosa
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf
first_indexed	2023-09-18T20:05:16Z
last_indexed	2023-09-18T20:05:16Z
_version_	1777407116719423488

In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa

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