The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies

The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was stud...

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Main Authors:	Nanda Earlia, Rahmad, Rahmad, Mohamad Amin, Prakoeswa, Crs, Khairan, Khairan, Idroes, Rinaldi
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf

id	ukm-13623
recordtype	eprints
spelling	ukm-136232019-11-10T04:08:43Z http://journalarticle.ukm.my/13623/ The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was studied by bioinformatics approach with reverse docking technique using palmitic acid as a control compound. For the stated purpose, two Pliek U extracts were prepared, namely ethanolic Pliek U extract (EPUE), and ethanolic of residue hexane of Pliek U extract (ERHPUE). The GC-MS results showed that lauric, myristic, palmitic, and oleic acids were predominant, followed by stearic, capric, linoleic, and caprylic acids. Reverse docking results showed that linoleic acid had the lowest binding affinity (-5.9 kcal/mol) and was strongly bound to E-FABP on the same side of amino acid GLN A98, ARG A81, TYR A22, and LYS A61. These findings indicated that linoleic acid has a potential utility as a drug candidate for atopic dermatitis treatment. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf Nanda Earlia, and Rahmad, Rahmad and Mohamad Amin, and Prakoeswa, Crs and Khairan, Khairan and Idroes, Rinaldi (2019) The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies. Sains Malaysiana, 48 (5). pp. 1019-1024. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was studied by bioinformatics approach with reverse docking technique using palmitic acid as a control compound. For the stated purpose, two Pliek U extracts were prepared, namely ethanolic Pliek U extract (EPUE), and ethanolic of residue hexane of Pliek U extract (ERHPUE). The GC-MS results showed that lauric, myristic, palmitic, and oleic acids were predominant, followed by stearic, capric, linoleic, and caprylic acids. Reverse docking results showed that linoleic acid had the lowest binding affinity (-5.9 kcal/mol) and was strongly bound to E-FABP on the same side of amino acid GLN A98, ARG A81, TYR A22, and LYS A61. These findings indicated that linoleic acid has a potential utility as a drug candidate for atopic dermatitis treatment.
format	Article
author	Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi
spellingShingle	Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
author_facet	Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi
author_sort	Nanda Earlia,
title	The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
title_short	The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
title_full	The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
title_fullStr	The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
title_full_unstemmed	The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
title_sort	potential effect of fatty acids from pliek u on epidermal fatty acid binding protein: chromatography and bioinformatic studies
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf
first_indexed	2023-09-18T20:05:16Z
last_indexed	2023-09-18T20:05:16Z
_version_	1777407115988566016

The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies

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