GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis

In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-...

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Main Authors:	Samina Khan Yusufzai, Mohammad Shaheen Khan, Eunice Lua Hanry, Mohd. Rafatullah, Belsheera Elison
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13621/ http://journalarticle.ukm.my/13621/ http://journalarticle.ukm.my/13621/1/08%20Samina%20Khan%20Yusufzai.pdf

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recordtype	eprints
spelling	ukm-136212019-11-10T03:41:47Z http://journalarticle.ukm.my/13621/ GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis Samina Khan Yusufzai, Mohammad Shaheen Khan, Eunice Lua Hanry, Mohd. Rafatullah, Belsheera Elison, In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-MS) analysis consuming hexane, chloroform and ethyl acetate as extraction solvents and gallic acid and quercetin as internal standards. In vitro antioxidant activity (AA) was determined by the application of 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay using tert-butyl-1-hydroxytoluene (BHT) as comparative drug. The GC-MS profiling showed the presence of 1-dodecanol (40.917%), oxalic acid, cyclobutyl octadecyl ester (24.985%), 1-octanol,2-nitro (12.424%), benzaldehyde, 2,4-dimethyl- (9.583%), 4-tridecanol (6.359%) and nitric acid, nonyl ester (5.616%) as major constituents. The TPC (224.62 ± 0.08 mg QE/g) and TFC (160.48 ± 0.08 mg GAE/g) was reported highest for the most polar solvent i.e. ethyl acetate. The in vitro antioxidant study disclosed highest IC50 value for ethyl acetate (2.15 mg/mL), queued by chloroform (1.19 mg/mL) and hexane (0.89 mg/mL), displaying that polar solvents are good extraction solvents for the identification of free radical scavenging properties, TFC and TPC. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13621/1/08%20Samina%20Khan%20Yusufzai.pdf Samina Khan Yusufzai, and Mohammad Shaheen Khan, and Eunice Lua Hanry, and Mohd. Rafatullah, and Belsheera Elison, (2019) GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis. Sains Malaysiana, 48 (5). pp. 999-1009. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-MS) analysis consuming hexane, chloroform and ethyl acetate as extraction solvents and gallic acid and quercetin as internal standards. In vitro antioxidant activity (AA) was determined by the application of 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay using tert-butyl-1-hydroxytoluene (BHT) as comparative drug. The GC-MS profiling showed the presence of 1-dodecanol (40.917%), oxalic acid, cyclobutyl octadecyl ester (24.985%), 1-octanol,2-nitro (12.424%), benzaldehyde, 2,4-dimethyl- (9.583%), 4-tridecanol (6.359%) and nitric acid, nonyl ester (5.616%) as major constituents. The TPC (224.62 ± 0.08 mg QE/g) and TFC (160.48 ± 0.08 mg GAE/g) was reported highest for the most polar solvent i.e. ethyl acetate. The in vitro antioxidant study disclosed highest IC50 value for ethyl acetate (2.15 mg/mL), queued by chloroform (1.19 mg/mL) and hexane (0.89 mg/mL), displaying that polar solvents are good extraction solvents for the identification of free radical scavenging properties, TFC and TPC.
format	Article
author	Samina Khan Yusufzai, Mohammad Shaheen Khan, Eunice Lua Hanry, Mohd. Rafatullah, Belsheera Elison,
spellingShingle	Samina Khan Yusufzai, Mohammad Shaheen Khan, Eunice Lua Hanry, Mohd. Rafatullah, Belsheera Elison, GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
author_facet	Samina Khan Yusufzai, Mohammad Shaheen Khan, Eunice Lua Hanry, Mohd. Rafatullah, Belsheera Elison,
author_sort	Samina Khan Yusufzai,
title	GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
title_short	GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
title_full	GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
title_fullStr	GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
title_full_unstemmed	GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
title_sort	gc-ms analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of bridelia stipularis
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13621/ http://journalarticle.ukm.my/13621/ http://journalarticle.ukm.my/13621/1/08%20Samina%20Khan%20Yusufzai.pdf
first_indexed	2023-09-18T20:05:16Z
last_indexed	2023-09-18T20:05:16Z
_version_	1777407115697061888

GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis

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