Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization

α-Amylase is the most extensively applied enzyme in industry. There is an urgent need for improvement on the yield of α-amylases currently. Herein, a strategy which combined Atmospheric and Room Temperature Plasma (ARTP) mutagenesis tool for construction of mutant library of Bacillus amyloliquefacie...

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Main Authors:	Xu, Ting-Liang, Jing, Peng, Zhu, Yu-Ling, Su, Li, Zhou, Kai-Yan, Cheng, Hai-Na, Tang, Shi-Zhe, Zhou, Hong-Bo
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13617/ http://journalarticle.ukm.my/13617/ http://journalarticle.ukm.my/13617/1/04%20Ting-liang%20Xu.pdf

id	ukm-13617
recordtype	eprints
spelling	ukm-136172019-11-10T03:22:08Z http://journalarticle.ukm.my/13617/ Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization Xu, Ting-Liang Jing, Peng Zhu, Yu-Ling Su, Li Zhou, Kai-Yan Cheng, Hai-Na Tang, Shi-Zhe Zhou, Hong-Bo α-Amylase is the most extensively applied enzyme in industry. There is an urgent need for improvement on the yield of α-amylases currently. Herein, a strategy which combined Atmospheric and Room Temperature Plasma (ARTP) mutagenesis tool for construction of mutant library of Bacillus amyloliquefaciens with a 24-well plates screening technique was adopted to improve the yield of recombinant Bacillus amyloliquefaciens α-amylases (BAA). A mutant strain named B. amyloliquefaciens ZN mut-7# was obtained, and the activity of BAA produced by this mutant strain was 86.92% higher than that of the original strain. B. amyloliquefaciens ZN mut-7# has an unchanged BAA gene and genetic stability. This successful application proved that ARTP can be applied to the genetically engineering strains that contain recombinant plasmid. Furthermore, response surface methodology offers an achievable and efficient strategy to optimize the composition of medium used to generate BAA in B. amyloliquefaciens ZN mut-7#. A 1.28-fold increase had been obtained compared to the production of non-optimized fermentation medium. This study demonstrates that ARTP mutagenesis and medium optimization are efficient and feasible methods for increasing recombinant enzyme production in the genetically engineering strains. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13617/1/04%20Ting-liang%20Xu.pdf Xu, Ting-Liang and Jing, Peng and Zhu, Yu-Ling and Su, Li and Zhou, Kai-Yan and Cheng, Hai-Na and Tang, Shi-Zhe and Zhou, Hong-Bo (2019) Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization. Sains Malaysiana, 48 (5). pp. 965-974. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	α-Amylase is the most extensively applied enzyme in industry. There is an urgent need for improvement on the yield of α-amylases currently. Herein, a strategy which combined Atmospheric and Room Temperature Plasma (ARTP) mutagenesis tool for construction of mutant library of Bacillus amyloliquefaciens with a 24-well plates screening technique was adopted to improve the yield of recombinant Bacillus amyloliquefaciens α-amylases (BAA). A mutant strain named B. amyloliquefaciens ZN mut-7# was obtained, and the activity of BAA produced by this mutant strain was 86.92% higher than that of the original strain. B. amyloliquefaciens ZN mut-7# has an unchanged BAA gene and genetic stability. This successful application proved that ARTP can be applied to the genetically engineering strains that contain recombinant plasmid. Furthermore, response surface methodology offers an achievable and efficient strategy to optimize the composition of medium used to generate BAA in B. amyloliquefaciens ZN mut-7#. A 1.28-fold increase had been obtained compared to the production of non-optimized fermentation medium. This study demonstrates that ARTP mutagenesis and medium optimization are efficient and feasible methods for increasing recombinant enzyme production in the genetically engineering strains.
format	Article
author	Xu, Ting-Liang Jing, Peng Zhu, Yu-Ling Su, Li Zhou, Kai-Yan Cheng, Hai-Na Tang, Shi-Zhe Zhou, Hong-Bo
spellingShingle	Xu, Ting-Liang Jing, Peng Zhu, Yu-Ling Su, Li Zhou, Kai-Yan Cheng, Hai-Na Tang, Shi-Zhe Zhou, Hong-Bo Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
author_facet	Xu, Ting-Liang Jing, Peng Zhu, Yu-Ling Su, Li Zhou, Kai-Yan Cheng, Hai-Na Tang, Shi-Zhe Zhou, Hong-Bo
author_sort	Xu, Ting-Liang
title	Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
title_short	Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
title_full	Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
title_fullStr	Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
title_full_unstemmed	Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization
title_sort	yield enhancement of recombinant α-amylases in bacillus amyloliquefaciens by artp mutagenesis-screening and medium optimization
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13617/ http://journalarticle.ukm.my/13617/ http://journalarticle.ukm.my/13617/1/04%20Ting-liang%20Xu.pdf
first_indexed	2023-09-18T20:05:15Z
last_indexed	2023-09-18T20:05:15Z
_version_	1777407115114053632

Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization

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