Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells

Zingiber zerumbet is widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. This study evaluated in vitro antioxidant potential of Z. zerumbet rhizome ethyl acetate extract on hydrogen peroxide (H2O2)-induced red blood cells (RBCs) damage; by measur...

Full description

Bibliographic Details
Main Authors:	Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13381/ http://journalarticle.ukm.my/13381/ http://journalarticle.ukm.my/13381/1/10%20Mohd%20Fuad%20Rahmat%20Sam.pdf

id	ukm-13381
recordtype	eprints
spelling	ukm-133812019-09-19T22:10:38Z http://journalarticle.ukm.my/13381/ Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin, Zingiber zerumbet is widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. This study evaluated in vitro antioxidant potential of Z. zerumbet rhizome ethyl acetate extract on hydrogen peroxide (H2O2)-induced red blood cells (RBCs) damage; by measuring percentage of haemolysis, oxidative damage and morphological changes in treated RBCs. On a preliminary dose-response analysis, it was observed that incubation of RBCs with extract at doses of 50, 25, 12.5 and 6.25 μg/mL all had no significant effect on RBCs similar to NaCl control and BHT (positive control), therefore, these concentrations were used in the subsequent study. The RBCs were pre-incubated with extract in the chosen concentrations, prior to treatment with H2O2. The results showed that only 6.25 μg/mL+H2O2 group showed significantly (p<0.05) lower percentage of haemolysis and oxidative damage; indicated by low level of malondialdehyde (MDA) and protein carbonyls (PC) compared to the H2O2 alone group. Electron microscopic examination further showed that pre-treatment with 6.25 μg/mL extract reduced the H2O2 –induced morphological changes of RBCs. Phytochemical analysis of extract using Gas Chromatography Mass Spectrometry (GCMS) identified Zerumbone as the highest constituent compound (51.57%) in this extract. In conclusion, this study indicated that 6.25 μg/mL ethyl acetate extract Z. zerumbet rhizome could efficiently protect RBCs against oxidative damage induced by H2O2 and this effects could possibly due the high constituent of Zerumbone. Penerbit Universiti Kebangsaan Malaysia 2019-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13381/1/10%20Mohd%20Fuad%20Rahmat%20Sam.pdf Mohd Fuad Rahmat Sam, and Asmah Hamid, and Ahmad Rohi Ghazali, and Santhana Raj Louis, and Siti Balkis Budin, (2019) Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells. Sains Malaysiana, 48 (4). pp. 781-790. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil4_2019/KandunganJilid48Bil4_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Zingiber zerumbet is widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. This study evaluated in vitro antioxidant potential of Z. zerumbet rhizome ethyl acetate extract on hydrogen peroxide (H2O2)-induced red blood cells (RBCs) damage; by measuring percentage of haemolysis, oxidative damage and morphological changes in treated RBCs. On a preliminary dose-response analysis, it was observed that incubation of RBCs with extract at doses of 50, 25, 12.5 and 6.25 μg/mL all had no significant effect on RBCs similar to NaCl control and BHT (positive control), therefore, these concentrations were used in the subsequent study. The RBCs were pre-incubated with extract in the chosen concentrations, prior to treatment with H2O2. The results showed that only 6.25 μg/mL+H2O2 group showed significantly (p<0.05) lower percentage of haemolysis and oxidative damage; indicated by low level of malondialdehyde (MDA) and protein carbonyls (PC) compared to the H2O2 alone group. Electron microscopic examination further showed that pre-treatment with 6.25 μg/mL extract reduced the H2O2 –induced morphological changes of RBCs. Phytochemical analysis of extract using Gas Chromatography Mass Spectrometry (GCMS) identified Zerumbone as the highest constituent compound (51.57%) in this extract. In conclusion, this study indicated that 6.25 μg/mL ethyl acetate extract Z. zerumbet rhizome could efficiently protect RBCs against oxidative damage induced by H2O2 and this effects could possibly due the high constituent of Zerumbone.
format	Article
author	Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin,
spellingShingle	Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin, Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
author_facet	Mohd Fuad Rahmat Sam, Asmah Hamid, Ahmad Rohi Ghazali, Santhana Raj Louis, Siti Balkis Budin,
author_sort	Mohd Fuad Rahmat Sam,
title	Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
title_short	Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
title_full	Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
title_fullStr	Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
title_full_unstemmed	Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
title_sort	protective effects of zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13381/ http://journalarticle.ukm.my/13381/ http://journalarticle.ukm.my/13381/1/10%20Mohd%20Fuad%20Rahmat%20Sam.pdf
first_indexed	2023-09-18T20:04:44Z
last_indexed	2023-09-18T20:04:44Z
_version_	1777407082267410432

Protective effects of Zingiber zerumbet ethyl acetate extract on hydrogen peroxide-induced damage of red blood cells

Similar Items