Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification

Sex determination is one of the basic components in victim identification. This study aims to ascertain the sex of an individual from burnt teeth samples exposed at different temperature and time through nested polymerase chain reaction (PCR) on the amelogenin (AMEL) sex marker, to calculate the spe...

Full description

Bibliographic Details
Main Authors:	Lim, Jonathan Jun-Yong, Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13343/ http://journalarticle.ukm.my/13343/ http://journalarticle.ukm.my/13343/1/23888-92207-1-PB.pdf

id	ukm-13343
recordtype	eprints
spelling	ukm-133432019-09-06T22:18:17Z http://journalarticle.ukm.my/13343/ Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification Lim, Jonathan Jun-Yong Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid, Sex determination is one of the basic components in victim identification. This study aims to ascertain the sex of an individual from burnt teeth samples exposed at different temperature and time through nested polymerase chain reaction (PCR) on the amelogenin (AMEL) sex marker, to calculate the specificity and sensitivity, and to compare with previous relevant studies. A total of 17 teeth samples was subjected to burning at different temperatures ranging from 100°C to 500°C, at 2 to 10 minutes. The whole tooth was used for deoxyribonucleic acid (DNA) extraction by phenol-chloroform method. All samples were quantified for DNA concentration and then analyzed with nested PCR using two pairs of AMEL primer and results of sex typing were recorded. Out of 17 samples, genomic DNA extracted from 6 samples have concentrations ranging from 27.3 – 130.6 ng/μL. Nested PCR could amplify 16 samples for AMEL gene. Sex typing using AMEL gene showed 76.47% accuracy. Sensitivity of AMEL primer was increased from 6.67% to 63.64% using nested PCR technique; specificity of both external and internal primer was reported at 100%. Nested PCR of AMEL gene proved to be a suitable method for unequivocal determination of sex from degraded DNA samples. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13343/1/23888-92207-1-PB.pdf Lim, Jonathan Jun-Yong and Mohd Fadhli Khamis, and Nur Haslindawaty Abd Rashid, (2019) Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification. Jurnal Sains Kesihatan Malaysia, 17 (1). pp. 91-98. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/686
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Sex determination is one of the basic components in victim identification. This study aims to ascertain the sex of an individual from burnt teeth samples exposed at different temperature and time through nested polymerase chain reaction (PCR) on the amelogenin (AMEL) sex marker, to calculate the specificity and sensitivity, and to compare with previous relevant studies. A total of 17 teeth samples was subjected to burning at different temperatures ranging from 100°C to 500°C, at 2 to 10 minutes. The whole tooth was used for deoxyribonucleic acid (DNA) extraction by phenol-chloroform method. All samples were quantified for DNA concentration and then analyzed with nested PCR using two pairs of AMEL primer and results of sex typing were recorded. Out of 17 samples, genomic DNA extracted from 6 samples have concentrations ranging from 27.3 – 130.6 ng/μL. Nested PCR could amplify 16 samples for AMEL gene. Sex typing using AMEL gene showed 76.47% accuracy. Sensitivity of AMEL primer was increased from 6.67% to 63.64% using nested PCR technique; specificity of both external and internal primer was reported at 100%. Nested PCR of AMEL gene proved to be a suitable method for unequivocal determination of sex from degraded DNA samples.
format	Article
author	Lim, Jonathan Jun-Yong Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid,
spellingShingle	Lim, Jonathan Jun-Yong Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid, Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
author_facet	Lim, Jonathan Jun-Yong Mohd Fadhli Khamis, Nur Haslindawaty Abd Rashid,
author_sort	Lim, Jonathan Jun-Yong
title	Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
title_short	Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
title_full	Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
title_fullStr	Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
title_full_unstemmed	Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
title_sort	identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13343/ http://journalarticle.ukm.my/13343/ http://journalarticle.ukm.my/13343/1/23888-92207-1-PB.pdf
first_indexed	2023-09-18T20:04:38Z
last_indexed	2023-09-18T20:04:38Z
_version_	1777407076679548928

Identification of amelogenin gene on burnt teeth samples through nested polymerase chain reaction amplification for sex identification

Similar Items