Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens

The controversial usage of antiseptic in treating wound infections had become a huge issue over the years due to its minimal effectiveness and high toxicity level that are harmful to humans. Hence, numerous studies had been carried out to determine other possible approaches including herbal remedies...

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Main Authors:	Hartini Yusof, Nur Aziatul Syakila Mohamad Radzi, Reena Leeba Richard
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13340/ http://journalarticle.ukm.my/13340/ http://journalarticle.ukm.my/13340/1/17066-92204-1-PB.pdf

id	ukm-13340
recordtype	eprints
spelling	ukm-133402019-09-06T22:10:29Z http://journalarticle.ukm.my/13340/ Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens Hartini Yusof, Nur Aziatul Syakila Mohamad Radzi, Reena Leeba Richard, The controversial usage of antiseptic in treating wound infections had become a huge issue over the years due to its minimal effectiveness and high toxicity level that are harmful to humans. Hence, numerous studies had been carried out to determine other possible approaches including herbal remedies. The alarming situation had led us to study on Piper sarmentosum and its antimicrobial activity against selected pathogens. In present study, methanol extract of Piper sarmentosum leaves were prepared to investigate the presence of phytochemical compounds. The in vitro antimicrobial activity of crude methanolic extract was evaluated using disc diffusion and microdilution broth methods. The study demonstrated the antimicrobial activity of leaves extract against Staphylococcus aureus (7 mm) and Escherichia coli (6.5 mm). However, no zone of inhibition was observed against Pseudomonas aeruginosa. Meanwhile, the MIC values for Staphylococcus aureus was 6.25 mg/mL whilst Escherichia coli was 12.5 mg/mL. In addition, the phytochemical screening results revealed that the extract contained glycosides, flavonoids, terpenoids, alkaloids and phenolics. In conclusion, methanolic extract of Piper sarmentosum leaves has the potential as a new, effective alternative towards current drugs that are available for skin-associated infection. The findings from this study are crucial in providing latest information of the plant’s additional values that can be incorporated as a baseline for current and future studies as well as in investigating other possible plants that are beneficial for health purposes, particularly for combating skin-associated infection. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13340/1/17066-92204-1-PB.pdf Hartini Yusof, and Nur Aziatul Syakila Mohamad Radzi, and Reena Leeba Richard, (2019) Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens. Jurnal Sains Kesihatan Malaysia, 17 (1). pp. 67-72. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/686
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The controversial usage of antiseptic in treating wound infections had become a huge issue over the years due to its minimal effectiveness and high toxicity level that are harmful to humans. Hence, numerous studies had been carried out to determine other possible approaches including herbal remedies. The alarming situation had led us to study on Piper sarmentosum and its antimicrobial activity against selected pathogens. In present study, methanol extract of Piper sarmentosum leaves were prepared to investigate the presence of phytochemical compounds. The in vitro antimicrobial activity of crude methanolic extract was evaluated using disc diffusion and microdilution broth methods. The study demonstrated the antimicrobial activity of leaves extract against Staphylococcus aureus (7 mm) and Escherichia coli (6.5 mm). However, no zone of inhibition was observed against Pseudomonas aeruginosa. Meanwhile, the MIC values for Staphylococcus aureus was 6.25 mg/mL whilst Escherichia coli was 12.5 mg/mL. In addition, the phytochemical screening results revealed that the extract contained glycosides, flavonoids, terpenoids, alkaloids and phenolics. In conclusion, methanolic extract of Piper sarmentosum leaves has the potential as a new, effective alternative towards current drugs that are available for skin-associated infection. The findings from this study are crucial in providing latest information of the plant’s additional values that can be incorporated as a baseline for current and future studies as well as in investigating other possible plants that are beneficial for health purposes, particularly for combating skin-associated infection.
format	Article
author	Hartini Yusof, Nur Aziatul Syakila Mohamad Radzi, Reena Leeba Richard,
spellingShingle	Hartini Yusof, Nur Aziatul Syakila Mohamad Radzi, Reena Leeba Richard, Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
author_facet	Hartini Yusof, Nur Aziatul Syakila Mohamad Radzi, Reena Leeba Richard,
author_sort	Hartini Yusof,
title	Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
title_short	Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
title_full	Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
title_fullStr	Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
title_full_unstemmed	Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens
title_sort	qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against selected pathogens
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13340/ http://journalarticle.ukm.my/13340/ http://journalarticle.ukm.my/13340/1/17066-92204-1-PB.pdf
first_indexed	2023-09-18T20:04:38Z
last_indexed	2023-09-18T20:04:38Z
_version_	1777407076233904128

Qualitative phytochemical analysis and antimicrobial activity of Piper sarmentosum leaves extract against selected pathogens

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