Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri

A characterization study of thin film gold electrode was performed. The electrode was fabricated using d. c. sputtering technique and analysis was conducted by dip-in method. A microliter plate well was used as the cell for this system. Test was done at room temperature where the exact temperatur...

Full description

Bibliographic Details
Main Authors:	Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar
Format:	Article
Published:	1997
Online Access:	http://journalarticle.ukm.my/1333/ http://journalarticle.ukm.my/1333/

id	ukm-1333
recordtype	eprints
spelling	ukm-13332011-10-11T03:45:18Z http://journalarticle.ukm.my/1333/ Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar, A characterization study of thin film gold electrode was performed. The electrode was fabricated using d. c. sputtering technique and analysis was conducted by dip-in method. A microliter plate well was used as the cell for this system. Test was done at room temperature where the exact temperature was entered manually and corrected by conductivity meter (WTW model 539) at a reference temperature of 25°C. The electrode has a good reproducible characteristic (p < 0.05) with response time of one minute and recovery time of one to three minutes. Its application as an analytical tool was shown in determination of Michaelis constants in enzyme kinetic study. Urease was chosen as a model enzyme and urea was used as a substrate. Km value obtained was 3.46 mM and Vmax was 1.930 μScm-1/min 1997 Article PeerReviewed Engku Norbaya Engku Muda, and Burhanuddin Yeop Majlis, and Mohd Aman Abu Bakar, (1997) Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri. Jurnal Kejuruteraan, 9 . http://www.ukm.my/jkukm/index.php/jkukm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
description	A characterization study of thin film gold electrode was performed. The electrode was fabricated using d. c. sputtering technique and analysis was conducted by dip-in method. A microliter plate well was used as the cell for this system. Test was done at room temperature where the exact temperature was entered manually and corrected by conductivity meter (WTW model 539) at a reference temperature of 25°C. The electrode has a good reproducible characteristic (p < 0.05) with response time of one minute and recovery time of one to three minutes. Its application as an analytical tool was shown in determination of Michaelis constants in enzyme kinetic study. Urease was chosen as a model enzyme and urea was used as a substrate. Km value obtained was 3.46 mM and Vmax was 1.930 μScm-1/min
format	Article
author	Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar,
spellingShingle	Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar, Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
author_facet	Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar,
author_sort	Engku Norbaya Engku Muda,
title	Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
title_short	Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
title_full	Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
title_fullStr	Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
title_full_unstemmed	Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
title_sort	penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
publishDate	1997
url	http://journalarticle.ukm.my/1333/ http://journalarticle.ukm.my/1333/
first_indexed	2023-09-18T19:33:04Z
last_indexed	2023-09-18T19:33:04Z
_version_	1777405089913241600

Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri

Similar Items