Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri
A characterization study of thin film gold electrode was performed. The electrode was fabricated using d. c. sputtering technique and analysis was conducted by dip-in method. A microliter plate well was used as the cell for this system. Test was done at room temperature where the exact temperatur...
| Main Authors: | , , |
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| Format: | Article |
| Published: |
1997
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| Online Access: | http://journalarticle.ukm.my/1333/ http://journalarticle.ukm.my/1333/ |
| Summary: | A characterization study of thin film gold electrode was performed. The electrode was
fabricated using d. c. sputtering technique and analysis was conducted by dip-in
method. A microliter plate well was used as the cell for this system. Test was done at
room temperature where the exact temperature was entered manually and corrected
by conductivity meter (WTW model 539) at a reference temperature of 25°C. The
electrode has a good reproducible characteristic (p < 0.05) with response time of one
minute and recovery time of one to three minutes. Its application as an analytical tool
was shown in determination of Michaelis constants in enzyme kinetic study. Urease
was chosen as a model enzyme and urea was used as a substrate. Km value obtained
was 3.46 mM and Vmax was 1.930 μScm-1/min |
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