Successful and efficient DNA extraction from processed pangolin scales

A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by d...

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Main Authors:	Xuan, Cai, Ye, Hao-Ting, Tian, En-Wei, Fang, Li, Chan, Li, Yang, Yao-Jun, Liu, Chun-Sheng, Zhi, Chao
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13313/ http://journalarticle.ukm.my/13313/ http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf

id	ukm-13313
recordtype	eprints
spelling	ukm-133132019-08-29T21:28:04Z http://journalarticle.ukm.my/13313/ Successful and efficient DNA extraction from processed pangolin scales Xuan, Cai Ye, Hao-Ting Tian, En-Wei Fang, Li Chan, Li Yang, Yao-Jun Liu, Chun-Sheng Zhi, Chao A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by digestion with proteinase K at 55°C for 120 h. Phenol-chloroform extraction was used to obtain the total DNA. PCR amplification for mitochondrial cytochrome b (cytb) gene was successful using the extracted DNA as the template, and sequencing of the amplified fragments confirmed Manis origin of the scale samples. With an efficiency up to 100%, this method is expected to provide a powerful tool in molecular identification of processed as well as crude pangolin scales. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf Xuan, Cai and Ye, Hao-Ting and Tian, En-Wei and Fang, Li and Chan, Li and Yang, Yao-Jun and Liu, Chun-Sheng and Zhi, Chao (2019) Successful and efficient DNA extraction from processed pangolin scales. Sains Malaysiana, 48 (3). pp. 555-559. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by digestion with proteinase K at 55°C for 120 h. Phenol-chloroform extraction was used to obtain the total DNA. PCR amplification for mitochondrial cytochrome b (cytb) gene was successful using the extracted DNA as the template, and sequencing of the amplified fragments confirmed Manis origin of the scale samples. With an efficiency up to 100%, this method is expected to provide a powerful tool in molecular identification of processed as well as crude pangolin scales.
format	Article
author	Xuan, Cai Ye, Hao-Ting Tian, En-Wei Fang, Li Chan, Li Yang, Yao-Jun Liu, Chun-Sheng Zhi, Chao
spellingShingle	Xuan, Cai Ye, Hao-Ting Tian, En-Wei Fang, Li Chan, Li Yang, Yao-Jun Liu, Chun-Sheng Zhi, Chao Successful and efficient DNA extraction from processed pangolin scales
author_facet	Xuan, Cai Ye, Hao-Ting Tian, En-Wei Fang, Li Chan, Li Yang, Yao-Jun Liu, Chun-Sheng Zhi, Chao
author_sort	Xuan, Cai
title	Successful and efficient DNA extraction from processed pangolin scales
title_short	Successful and efficient DNA extraction from processed pangolin scales
title_full	Successful and efficient DNA extraction from processed pangolin scales
title_fullStr	Successful and efficient DNA extraction from processed pangolin scales
title_full_unstemmed	Successful and efficient DNA extraction from processed pangolin scales
title_sort	successful and efficient dna extraction from processed pangolin scales
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13313/ http://journalarticle.ukm.my/13313/ http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf
first_indexed	2023-09-18T20:04:34Z
last_indexed	2023-09-18T20:04:34Z
_version_	1777407072449593344

Successful and efficient DNA extraction from processed pangolin scales

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