Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory

The altered molecular mechanisms by experimental therapies for neurodegenerative diseases itself could be overlapped with genes induced by the behavioral task. We employed the microarray platform to identify the differentially expressed late responsive genes in medial temporal lobes of four Morris W...

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Main Authors:	Teh, Hui-Xin, Then, Sue-Mian, Indah Maisarah Shani, Musalmah Mazlan, Wan Zurinah Wan Ngah, Rahman Jamal
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/13236/ http://journalarticle.ukm.my/13236/ http://journalarticle.ukm.my/13236/1/6-10-1-SM.pdf

id	ukm-13236
recordtype	eprints
spelling	ukm-132362019-07-29T04:19:42Z http://journalarticle.ukm.my/13236/ Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory Teh, Hui-Xin Then, Sue-Mian Indah Maisarah Shani, Musalmah Mazlan, Wan Zurinah Wan Ngah, Rahman Jamal, The altered molecular mechanisms by experimental therapies for neurodegenerative diseases itself could be overlapped with genes induced by the behavioral task. We employed the microarray platform to identify the differentially expressed late responsive genes in medial temporal lobes of four Morris Water Maze (MWM) trained and untrained BALB/c mice. After MWM training, the mice were sacrificed to obtain their brains’ medial temporal lobes. The total RNA was extracted from the tissues and global mRNA gene expression analysis was performed using Affymetrix GeneChip ®Mouse Gene 1.0 ST Array. There were 3635 (62.2%) up-regulated genes and 2206 (37.8%) down-regulated genes at p-values of < 0.05. These genes were operationally defined as late memory-related genes and behavior-related genes indicating that behavioral learning has a significant impact on the gene expression of the medial temporal lobes. From the pathway analysis, the network of memory and locomotion genes, and the guanylate cyclase pathway were identified as one of the most interesting pathways. The qPCR validation showed that the genes NMDA receptor 2a (Nmda2a) and cAMP dependent protein kinase type I beta regulatory subunit (Prkar1b) were up-regulated while adenylate cyclase 5 (Adcy5) was down-regulated. We proposed that the involvement of guanylate cyclase pathway in the long-term potentiation lasted at least up to three days after the MWM test. Present study suggested that the molecular mechanisms followed by spatial learning task could be altered up to three days or even longer, it could be overlapped with genes induced by further invented experimental therapy. Pusat Perubatan Universiti Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13236/1/6-10-1-SM.pdf Teh, Hui-Xin and Then, Sue-Mian and Indah Maisarah Shani, and Musalmah Mazlan, and Wan Zurinah Wan Ngah, and Rahman Jamal, (2013) Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory. Asia-Pacific Journal of Molecular Medicine, 3 (2). pp. 1-12. ISSN 2232-0326 http://spaj.ukm.my/apjmm/index.php/apjmm/issue/view/4
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The altered molecular mechanisms by experimental therapies for neurodegenerative diseases itself could be overlapped with genes induced by the behavioral task. We employed the microarray platform to identify the differentially expressed late responsive genes in medial temporal lobes of four Morris Water Maze (MWM) trained and untrained BALB/c mice. After MWM training, the mice were sacrificed to obtain their brains’ medial temporal lobes. The total RNA was extracted from the tissues and global mRNA gene expression analysis was performed using Affymetrix GeneChip ®Mouse Gene 1.0 ST Array. There were 3635 (62.2%) up-regulated genes and 2206 (37.8%) down-regulated genes at p-values of < 0.05. These genes were operationally defined as late memory-related genes and behavior-related genes indicating that behavioral learning has a significant impact on the gene expression of the medial temporal lobes. From the pathway analysis, the network of memory and locomotion genes, and the guanylate cyclase pathway were identified as one of the most interesting pathways. The qPCR validation showed that the genes NMDA receptor 2a (Nmda2a) and cAMP dependent protein kinase type I beta regulatory subunit (Prkar1b) were up-regulated while adenylate cyclase 5 (Adcy5) was down-regulated. We proposed that the involvement of guanylate cyclase pathway in the long-term potentiation lasted at least up to three days after the MWM test. Present study suggested that the molecular mechanisms followed by spatial learning task could be altered up to three days or even longer, it could be overlapped with genes induced by further invented experimental therapy.
format	Article
author	Teh, Hui-Xin Then, Sue-Mian Indah Maisarah Shani, Musalmah Mazlan, Wan Zurinah Wan Ngah, Rahman Jamal,
spellingShingle	Teh, Hui-Xin Then, Sue-Mian Indah Maisarah Shani, Musalmah Mazlan, Wan Zurinah Wan Ngah, Rahman Jamal, Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
author_facet	Teh, Hui-Xin Then, Sue-Mian Indah Maisarah Shani, Musalmah Mazlan, Wan Zurinah Wan Ngah, Rahman Jamal,
author_sort	Teh, Hui-Xin
title	Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
title_short	Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
title_full	Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
title_fullStr	Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
title_full_unstemmed	Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
title_sort	expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/13236/ http://journalarticle.ukm.my/13236/ http://journalarticle.ukm.my/13236/1/6-10-1-SM.pdf
first_indexed	2023-09-18T20:04:24Z
last_indexed	2023-09-18T20:04:24Z
_version_	1777407061811789824

Expression profile of late responsive genes induced by spatial learning task: involvement of pathways in locomotion and memory

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