Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients

Breast cancer is a serious health concern and still a leading cause of death among women in the world. To explore the complexity of this cancer, we performed microarray analysis on highly selective cancer and normal breast tissues. The aim of this study was to identify differentially expressed gen...

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Main Authors:	Chin, Minning, Norfilza Mohd Mokhtar, Ezanee Azlina Mohd Haniff, Norlia Abdullah, Rohaizak Muhammad, Nor Aina Emran, Siti Aishah Che Md Ali, Rahman Jamal
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/13234/ http://journalarticle.ukm.my/13234/ http://journalarticle.ukm.my/13234/1/4-8-1-SM.pdf

id	ukm-13234
recordtype	eprints
spelling	ukm-132342019-07-30T21:25:48Z http://journalarticle.ukm.my/13234/ Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients Chin, Minning Norfilza Mohd Mokhtar, Ezanee Azlina Mohd Haniff, Norlia Abdullah, Rohaizak Muhammad, Nor Aina Emran, Siti Aishah Che Md Ali, Rahman Jamal, Breast cancer is a serious health concern and still a leading cause of death among women in the world. To explore the complexity of this cancer, we performed microarray analysis on highly selective cancer and normal breast tissues. The aim of this study was to identify differentially expressed genes between both tissues and to elucidate further molecular pathways involved in breast cancer carcinogenesis. Genome-wide expression profiling was performed on fifteen cancer and five normal breast tissues using the Affymetrix GeneChip® Human Gene 1.0 ST array. Supervised hierarchical cluster analysis using filtering parameters of -1.5 to 1.5 fold-change and p-value with False Discovery Rate < 0.05 revealed 404 up-regulated and 463 down-regulated genes. Pathway analysis revealed the significant genes were involved in cell cycle regulation, DNA repair, Hedgehog pathway, histone phosphorylation, TRRAP/Tip60 chromatin remodelling and apoptosis regulation. Among the top 10 significantly overexpressed genes were CENPF, DTL and MK167 and these were related to cell cycle regulation. Among the top 10 significant down-regulated genes, HOXA5 and NRG1 were found to be associated with Wnt signalling pathway and ErbB signalling pathway respectively. Aberrations in these genes are likely to promote breast cancer carcinogenesis. Our current findings highlighted the importance of differentially expressed genes in breast cancer and their molecular pathways that linked these genes. Further studies are required to validate our findings using larger sample size. Pusat Perubatan Universiti Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13234/1/4-8-1-SM.pdf Chin, Minning and Norfilza Mohd Mokhtar, and Ezanee Azlina Mohd Haniff, and Norlia Abdullah, and Rohaizak Muhammad, and Nor Aina Emran, and Siti Aishah Che Md Ali, and Rahman Jamal, (2013) Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients. Asia-Pacific Journal of Molecular Medicine, 3 (1). pp. 1-7. ISSN 2232-0326 http://spaj.ukm.my/apjmm/index.php/apjmm/issue/view/3
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Breast cancer is a serious health concern and still a leading cause of death among women in the world. To explore the complexity of this cancer, we performed microarray analysis on highly selective cancer and normal breast tissues. The aim of this study was to identify differentially expressed genes between both tissues and to elucidate further molecular pathways involved in breast cancer carcinogenesis. Genome-wide expression profiling was performed on fifteen cancer and five normal breast tissues using the Affymetrix GeneChip® Human Gene 1.0 ST array. Supervised hierarchical cluster analysis using filtering parameters of -1.5 to 1.5 fold-change and p-value with False Discovery Rate < 0.05 revealed 404 up-regulated and 463 down-regulated genes. Pathway analysis revealed the significant genes were involved in cell cycle regulation, DNA repair, Hedgehog pathway, histone phosphorylation, TRRAP/Tip60 chromatin remodelling and apoptosis regulation. Among the top 10 significantly overexpressed genes were CENPF, DTL and MK167 and these were related to cell cycle regulation. Among the top 10 significant down-regulated genes, HOXA5 and NRG1 were found to be associated with Wnt signalling pathway and ErbB signalling pathway respectively. Aberrations in these genes are likely to promote breast cancer carcinogenesis. Our current findings highlighted the importance of differentially expressed genes in breast cancer and their molecular pathways that linked these genes. Further studies are required to validate our findings using larger sample size.
format	Article
author	Chin, Minning Norfilza Mohd Mokhtar, Ezanee Azlina Mohd Haniff, Norlia Abdullah, Rohaizak Muhammad, Nor Aina Emran, Siti Aishah Che Md Ali, Rahman Jamal,
spellingShingle	Chin, Minning Norfilza Mohd Mokhtar, Ezanee Azlina Mohd Haniff, Norlia Abdullah, Rohaizak Muhammad, Nor Aina Emran, Siti Aishah Che Md Ali, Rahman Jamal, Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
author_facet	Chin, Minning Norfilza Mohd Mokhtar, Ezanee Azlina Mohd Haniff, Norlia Abdullah, Rohaizak Muhammad, Nor Aina Emran, Siti Aishah Che Md Ali, Rahman Jamal,
author_sort	Chin, Minning
title	Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
title_short	Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
title_full	Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
title_fullStr	Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
title_full_unstemmed	Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients
title_sort	potential biomarkers through genome-wide expression analysis of breast cancer samples from malaysian patients
publisher	Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/13234/ http://journalarticle.ukm.my/13234/ http://journalarticle.ukm.my/13234/1/4-8-1-SM.pdf
first_indexed	2023-09-18T20:04:24Z
last_indexed	2023-09-18T20:04:24Z
_version_	1777407061516091392

Potential biomarkers through genome-wide expression analysis of breast cancer samples from Malaysian patients

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