The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model

The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model

Oleuropein is a phenolic compound that can be abundantly found in the olive plant and it possesses pharmacological properties including anticancer, antioxidant, and anti-inflammatory. This present study was designed to determine the effects of oleuropein on tumour promotion stage, particularly on th...

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Main Authors: Siti Fathiah Masre, Azim Izzuddeen, Dayang Noor Suzliana John, Zariyantey Abdul Hamid
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13086/
http://journalarticle.ukm.my/13086/
http://journalarticle.ukm.my/13086/1/11%20Siti%20Fathiah%20Masre.pdf
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recordtype eprints
spelling ukm-130862019-06-20T15:16:18Z http://journalarticle.ukm.my/13086/ The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model Siti Fathiah Masre, Azim Izzuddeen, Dayang Noor Suzliana John, Zariyantey Abdul Hamid, Oleuropein is a phenolic compound that can be abundantly found in the olive plant and it possesses pharmacological properties including anticancer, antioxidant, and anti-inflammatory. This present study was designed to determine the effects of oleuropein on tumour promotion stage, particularly on the histopathological changes, apoptotic rates and oxidative stress profiles by using the mouse skin carcinogenesis model. Female ICR mice were randomly divided into 3 groups (n= 8 mice per group) as follows: Control induced with DMBA/TPA, negative control (acetone) and oleuropein-treated groups. For the treatment group, the mice were initiated with DMBA (200 nmol) followed by pre-treatment with oleuropein (10 mg/kg) and subsequent promotion with TPA (20 nmol). The treatments were topically applied on the shaved dorsal up to 10 weeks. Histopathology analysis showed that oleuropein-pretreated group appeared lack of thickness in epidermal hyperplasia, as compared to thick hyperplasia and epidermal disorganisation in the DMBA/TPA control group. Data also showed that oleuropein pre-treatment resulted in a significant increase of the apoptotic rates (p<0.05) as indicated by the activated caspase-3 labelling compared to DMBA/TPA group. Interestingly, the level of MDA is significantly reduced (p<0.05) in the oleuropein pre-treated group compared to DMBA/TPA group. Next, pre-treatment of oleuropein caused a significant decrease in the GSH levels (p<0.05) along with a significant increase in the SOD levels (p<0.05) compared to the DMBA/TPA group. Overall, this study indicates that oleuropein may act as a potential chemopreventive agent through its apoptotic and antioxidant defence activities on tumour promotion stage in skin carcinogenesis event. Penerbit Universiti Kebangsaan Malaysia 2019-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13086/1/11%20Siti%20Fathiah%20Masre.pdf Siti Fathiah Masre, and Azim Izzuddeen, and Dayang Noor Suzliana John, and Zariyantey Abdul Hamid, (2019) The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model. Sains Malaysiana, 48 (2). pp. 347-352. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil2_2019/KandunganJilid48Bil2_2019.html
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institution Universiti Kebangasaan Malaysia
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language English
description Oleuropein is a phenolic compound that can be abundantly found in the olive plant and it possesses pharmacological properties including anticancer, antioxidant, and anti-inflammatory. This present study was designed to determine the effects of oleuropein on tumour promotion stage, particularly on the histopathological changes, apoptotic rates and oxidative stress profiles by using the mouse skin carcinogenesis model. Female ICR mice were randomly divided into 3 groups (n= 8 mice per group) as follows: Control induced with DMBA/TPA, negative control (acetone) and oleuropein-treated groups. For the treatment group, the mice were initiated with DMBA (200 nmol) followed by pre-treatment with oleuropein (10 mg/kg) and subsequent promotion with TPA (20 nmol). The treatments were topically applied on the shaved dorsal up to 10 weeks. Histopathology analysis showed that oleuropein-pretreated group appeared lack of thickness in epidermal hyperplasia, as compared to thick hyperplasia and epidermal disorganisation in the DMBA/TPA control group. Data also showed that oleuropein pre-treatment resulted in a significant increase of the apoptotic rates (p<0.05) as indicated by the activated caspase-3 labelling compared to DMBA/TPA group. Interestingly, the level of MDA is significantly reduced (p<0.05) in the oleuropein pre-treated group compared to DMBA/TPA group. Next, pre-treatment of oleuropein caused a significant decrease in the GSH levels (p<0.05) along with a significant increase in the SOD levels (p<0.05) compared to the DMBA/TPA group. Overall, this study indicates that oleuropein may act as a potential chemopreventive agent through its apoptotic and antioxidant defence activities on tumour promotion stage in skin carcinogenesis event.
format Article
author Siti Fathiah Masre,
Azim Izzuddeen,
Dayang Noor Suzliana John,
Zariyantey Abdul Hamid,
spellingShingle Siti Fathiah Masre,
Azim Izzuddeen,
Dayang Noor Suzliana John,
Zariyantey Abdul Hamid,
The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
author_facet Siti Fathiah Masre,
Azim Izzuddeen,
Dayang Noor Suzliana John,
Zariyantey Abdul Hamid,
author_sort Siti Fathiah Masre,
title The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
title_short The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
title_full The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
title_fullStr The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
title_full_unstemmed The effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
title_sort effects of oleuropein on apoptotic rate and oxidative stress profiles during tumour promotion stage in the mouse skin carcinogenesis model
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13086/
http://journalarticle.ukm.my/13086/
http://journalarticle.ukm.my/13086/1/11%20Siti%20Fathiah%20Masre.pdf
first_indexed 2023-09-18T20:04:04Z
last_indexed 2023-09-18T20:04:04Z
_version_ 1777407040353730560

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