Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea

Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In...

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Main Authors:	Jang, Min-Seok, Oh, Myung-Joo, Kim, Seok-Ryel, Kim, Wi-Sik
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13079/ http://journalarticle.ukm.my/13079/ http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf

id	ukm-13079
recordtype	eprints
spelling	ukm-130792019-06-20T15:25:28Z http://journalarticle.ukm.my/13079/ Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea Jang, Min-Seok Oh, Myung-Joo Kim, Seok-Ryel Kim, Wi-Sik Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In the present study, a survey was conducted to investigate VHSV infection in 39 olive flounder hatcheries from 2014 to 2017. Fish were tested for the presence of VHSV by inoculating sample to fathead minnow (FHM) and chinook salmon embryo (CHSE-214) cells to observe cytopathic effect, reverse transcription-polymerase chain reaction (RT-PCR), and antibody detection enzyme-linked immunosorbent assay (ELISA). VHSV was not detected in any of the 2,430 fish (from 461 pooled and 156 unpooled samples), although 34 (20.3%) of 167 samples was found to be positive for marine birnavirus (MABV) by cell culture and RT-PCR. Antibody detection ELISA results showed that all 212 fish sera have optical density (OD) values below of 0.1, suggesting that these fish had no VHSV-specific antibodies. Moreover, VHSV was not detected in any of 40 pooled samples (172 fish) collected after shifting rearing water temperature from 17-21°C to 10-15°C. In conclusion, the 39 olive flounder hatcheries surveyed in Korea was not infected by VHSV. Penerbit Universiti Kebangsaan Malaysia 2019-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf Jang, Min-Seok and Oh, Myung-Joo and Kim, Seok-Ryel and Kim, Wi-Sik (2019) Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea. Sains Malaysiana, 48 (2). pp. 291-299. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In the present study, a survey was conducted to investigate VHSV infection in 39 olive flounder hatcheries from 2014 to 2017. Fish were tested for the presence of VHSV by inoculating sample to fathead minnow (FHM) and chinook salmon embryo (CHSE-214) cells to observe cytopathic effect, reverse transcription-polymerase chain reaction (RT-PCR), and antibody detection enzyme-linked immunosorbent assay (ELISA). VHSV was not detected in any of the 2,430 fish (from 461 pooled and 156 unpooled samples), although 34 (20.3%) of 167 samples was found to be positive for marine birnavirus (MABV) by cell culture and RT-PCR. Antibody detection ELISA results showed that all 212 fish sera have optical density (OD) values below of 0.1, suggesting that these fish had no VHSV-specific antibodies. Moreover, VHSV was not detected in any of 40 pooled samples (172 fish) collected after shifting rearing water temperature from 17-21°C to 10-15°C. In conclusion, the 39 olive flounder hatcheries surveyed in Korea was not infected by VHSV.
format	Article
author	Jang, Min-Seok Oh, Myung-Joo Kim, Seok-Ryel Kim, Wi-Sik
spellingShingle	Jang, Min-Seok Oh, Myung-Joo Kim, Seok-Ryel Kim, Wi-Sik Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
author_facet	Jang, Min-Seok Oh, Myung-Joo Kim, Seok-Ryel Kim, Wi-Sik
author_sort	Jang, Min-Seok
title	Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_short	Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_full	Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_fullStr	Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_full_unstemmed	Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_sort	survey of viral hemorrhagic septicemia virus (vhsv) in olive flounder, paralichthys olivaceus hatchery in korea
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13079/ http://journalarticle.ukm.my/13079/ http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf
first_indexed	2023-09-18T20:04:03Z
last_indexed	2023-09-18T20:04:03Z
_version_	1777407039330320384

Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea

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