Long term effect of cryopreservation on primary human skin cells

Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with c...

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Main Authors:	Ishak M.F., Manira M., Ng, Min Hwei, Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13062/ http://journalarticle.ukm.my/13062/ http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf

id	ukm-13062
recordtype	eprints
spelling	ukm-130622019-06-20T14:39:36Z http://journalarticle.ukm.my/13062/ Long term effect of cryopreservation on primary human skin cells Ishak M.F., Manira M., Ng, Min Hwei Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus, Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with consent from patients. The tissue was cleaned, processed and cultured until passage 3. Upon confluency, cells were trypsinised and total cell yield and viability were determined before and after being cryopreserved. Sterility and immunocytochemistry analysis for collagen type I (Col-1) and cytokeratin 14 (CK14) antibodies were also performed on cells cryopreserved for one, three, six and twelve months. There is no significant difference in growth rates for cryopreserved cells for 1 to 12 months, except for fibroblasts at 6 months. Cell viability for both keratinocytes and fibroblasts decreased with time (65%± 3.5% - 89%± 4.5%). Sterility testing showed no contamination after 12 months of cryopreservation. Immunocytochemistry analysis showed positive expression for CK14 (keratinocytes) and Col -1 (fibroblasts) after 12 months of cryopreservation. Morphologically, keratinocytes and fibroblasts were able to retain its phenotype. The loss in viability is consistent in all samples and possibly due to thermal-cycling effect. Immunocytochemistry and consistent cell growth analysis showed that keratinocytes and fibroblasts were able to retain their characteristics in cryopreservation condition. These preliminary findings show that primary skin cells can be stored via cryopreservation and still retain their characteristics. However, further investigations using longer periods of cryopreservation (24 months, 48 months) should be conducted. Penerbit Universiti Kebangsaan Malaysia 2019-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf Ishak M.F., and Manira M., and Ng, Min Hwei and Khairul B., and Gargy L., and Aminuddin B.S., and Ruszymah Haji Idrus, (2019) Long term effect of cryopreservation on primary human skin cells. Sains Malaysiana, 48 (1). pp. 137-144. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with consent from patients. The tissue was cleaned, processed and cultured until passage 3. Upon confluency, cells were trypsinised and total cell yield and viability were determined before and after being cryopreserved. Sterility and immunocytochemistry analysis for collagen type I (Col-1) and cytokeratin 14 (CK14) antibodies were also performed on cells cryopreserved for one, three, six and twelve months. There is no significant difference in growth rates for cryopreserved cells for 1 to 12 months, except for fibroblasts at 6 months. Cell viability for both keratinocytes and fibroblasts decreased with time (65%± 3.5% - 89%± 4.5%). Sterility testing showed no contamination after 12 months of cryopreservation. Immunocytochemistry analysis showed positive expression for CK14 (keratinocytes) and Col -1 (fibroblasts) after 12 months of cryopreservation. Morphologically, keratinocytes and fibroblasts were able to retain its phenotype. The loss in viability is consistent in all samples and possibly due to thermal-cycling effect. Immunocytochemistry and consistent cell growth analysis showed that keratinocytes and fibroblasts were able to retain their characteristics in cryopreservation condition. These preliminary findings show that primary skin cells can be stored via cryopreservation and still retain their characteristics. However, further investigations using longer periods of cryopreservation (24 months, 48 months) should be conducted.
format	Article
author	Ishak M.F., Manira M., Ng, Min Hwei Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus,
spellingShingle	Ishak M.F., Manira M., Ng, Min Hwei Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus, Long term effect of cryopreservation on primary human skin cells
author_facet	Ishak M.F., Manira M., Ng, Min Hwei Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus,
author_sort	Ishak M.F.,
title	Long term effect of cryopreservation on primary human skin cells
title_short	Long term effect of cryopreservation on primary human skin cells
title_full	Long term effect of cryopreservation on primary human skin cells
title_fullStr	Long term effect of cryopreservation on primary human skin cells
title_full_unstemmed	Long term effect of cryopreservation on primary human skin cells
title_sort	long term effect of cryopreservation on primary human skin cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13062/ http://journalarticle.ukm.my/13062/ http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf
first_indexed	2023-09-18T20:04:00Z
last_indexed	2023-09-18T20:04:00Z
_version_	1777407036875603968

Long term effect of cryopreservation on primary human skin cells

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