Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract

Zingiber zerumbet is a plant that is traditionally consumed in many countries, including Malaysia due to its therapeutic properties. Previously it has been proven to inhibit migration and proliferation of cancer cells. However, its effects on normal cells are still unknown and would be the main focu...

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Main Authors:	Mazlyzam Abdul Latif, Farah Wahida Ibrahim, Siti Aisyah Arshad, Chua, Kien Hui, Nurul Farhana Jufri, Asmah Hamid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13060/ http://journalarticle.ukm.my/13060/ http://journalarticle.ukm.my/13060/1/14%20Mazlyzam%20Abdul%20Latif.pdf

id	ukm-13060
recordtype	eprints
spelling	ukm-130602019-06-20T14:37:04Z http://journalarticle.ukm.my/13060/ Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract Mazlyzam Abdul Latif, Farah Wahida Ibrahim, Siti Aisyah Arshad, Chua, Kien Hui Nurul Farhana Jufri, Asmah Hamid, Zingiber zerumbet is a plant that is traditionally consumed in many countries, including Malaysia due to its therapeutic properties. Previously it has been proven to inhibit migration and proliferation of cancer cells. However, its effects on normal cells are still unknown and would be the main focus of this study. The cytotoxicity along with proliferation and migration activities were evaluated against human dermal fibroblast adult cell line (HDF-a) using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium], proliferation and scratch assays. The cytotoxicity effect was determined following 24 h of treatment by ethyl acetate Z. zerumbet extracts while proliferation assay was conducted at different concentrations (500, 750, 1000, 1250 and 1500 μg/mL) at 24, 48 and 72 h. The cell migration rate was determined by scratch assay in 6-well culture plate using 10 μL pipette tip following 80% confluency at optimum concentration (750 μg/mL) of Z. zerumbet at different time points. Migration rate was scored at every 24 h using digital camera connected to the inverted microscope. The results from cytotoxicity test showed no IC50 values were observed for Z. zerumbet extract as compared to the positive control (menadione). Proliferation assay exhibited highest cell viability (137%) at 750 μg/mL. The rate of cell migration increased following treatment with Z. zerumbet extract after 48 h. However, the result was not significantly different when compared to the control. In conclusion, Z. zerumbet ethyl acetate extract is able to induce cell proliferation and potentially promoting migration rate of wound healing in in-vitro model. Penerbit Universiti Kebangsaan Malaysia 2019-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13060/1/14%20Mazlyzam%20Abdul%20Latif.pdf Mazlyzam Abdul Latif, and Farah Wahida Ibrahim, and Siti Aisyah Arshad, and Chua, Kien Hui and Nurul Farhana Jufri, and Asmah Hamid, (2019) Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract. Sains Malaysiana, 48 (1). pp. 121-127. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Zingiber zerumbet is a plant that is traditionally consumed in many countries, including Malaysia due to its therapeutic properties. Previously it has been proven to inhibit migration and proliferation of cancer cells. However, its effects on normal cells are still unknown and would be the main focus of this study. The cytotoxicity along with proliferation and migration activities were evaluated against human dermal fibroblast adult cell line (HDF-a) using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium], proliferation and scratch assays. The cytotoxicity effect was determined following 24 h of treatment by ethyl acetate Z. zerumbet extracts while proliferation assay was conducted at different concentrations (500, 750, 1000, 1250 and 1500 μg/mL) at 24, 48 and 72 h. The cell migration rate was determined by scratch assay in 6-well culture plate using 10 μL pipette tip following 80% confluency at optimum concentration (750 μg/mL) of Z. zerumbet at different time points. Migration rate was scored at every 24 h using digital camera connected to the inverted microscope. The results from cytotoxicity test showed no IC50 values were observed for Z. zerumbet extract as compared to the positive control (menadione). Proliferation assay exhibited highest cell viability (137%) at 750 μg/mL. The rate of cell migration increased following treatment with Z. zerumbet extract after 48 h. However, the result was not significantly different when compared to the control. In conclusion, Z. zerumbet ethyl acetate extract is able to induce cell proliferation and potentially promoting migration rate of wound healing in in-vitro model.
format	Article
author	Mazlyzam Abdul Latif, Farah Wahida Ibrahim, Siti Aisyah Arshad, Chua, Kien Hui Nurul Farhana Jufri, Asmah Hamid,
spellingShingle	Mazlyzam Abdul Latif, Farah Wahida Ibrahim, Siti Aisyah Arshad, Chua, Kien Hui Nurul Farhana Jufri, Asmah Hamid, Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
author_facet	Mazlyzam Abdul Latif, Farah Wahida Ibrahim, Siti Aisyah Arshad, Chua, Kien Hui Nurul Farhana Jufri, Asmah Hamid,
author_sort	Mazlyzam Abdul Latif,
title	Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
title_short	Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
title_full	Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
title_fullStr	Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
title_full_unstemmed	Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
title_sort	cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using zingiber zerumbet extract
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13060/ http://journalarticle.ukm.my/13060/ http://journalarticle.ukm.my/13060/1/14%20Mazlyzam%20Abdul%20Latif.pdf
first_indexed	2023-09-18T20:04:00Z
last_indexed	2023-09-18T20:04:00Z
_version_	1777407036576759808

Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract

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