Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology

The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the obj...

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Main Authors:	Sudarat Suwannarat, Jutarut Iewkittayakorn, Yaowapa Sukpondma, Vatcharin Rukachaisirikul, Souwalak Phongpaichit, Wilaiwan Chotigeat
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13057/ http://journalarticle.ukm.my/13057/ http://journalarticle.ukm.my/13057/1/11%20Sudarat%20Suwannarat.pdf

id	ukm-13057
recordtype	eprints
spelling	ukm-130572019-06-20T15:13:51Z http://journalarticle.ukm.my/13057/ Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology Sudarat Suwannarat, Jutarut Iewkittayakorn, Yaowapa Sukpondma, Vatcharin Rukachaisirikul, Souwalak Phongpaichit, Wilaiwan Chotigeat, The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield. Penerbit Universiti Kebangsaan Malaysia 2019-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13057/1/11%20Sudarat%20Suwannarat.pdf Sudarat Suwannarat, and Jutarut Iewkittayakorn, and Yaowapa Sukpondma, and Vatcharin Rukachaisirikul, and Souwalak Phongpaichit, and Wilaiwan Chotigeat, (2019) Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology. Sains Malaysiana, 48 (1). pp. 93-102. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield.
format	Article
author	Sudarat Suwannarat, Jutarut Iewkittayakorn, Yaowapa Sukpondma, Vatcharin Rukachaisirikul, Souwalak Phongpaichit, Wilaiwan Chotigeat,
spellingShingle	Sudarat Suwannarat, Jutarut Iewkittayakorn, Yaowapa Sukpondma, Vatcharin Rukachaisirikul, Souwalak Phongpaichit, Wilaiwan Chotigeat, Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
author_facet	Sudarat Suwannarat, Jutarut Iewkittayakorn, Yaowapa Sukpondma, Vatcharin Rukachaisirikul, Souwalak Phongpaichit, Wilaiwan Chotigeat,
author_sort	Sudarat Suwannarat,
title	Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
title_short	Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
title_full	Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
title_fullStr	Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
title_full_unstemmed	Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology
title_sort	optimization of the production of lovastatin from aspergillus sclerotiorum psu-rspg178 under static liquid culture using response surface methodology
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13057/ http://journalarticle.ukm.my/13057/ http://journalarticle.ukm.my/13057/1/11%20Sudarat%20Suwannarat.pdf
first_indexed	2023-09-18T20:04:00Z
last_indexed	2023-09-18T20:04:00Z
_version_	1777407036121677824

Optimization of the production of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 under static liquid culture using response surface methodology

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