Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice

In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant in...

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Main Authors:	Lee, Yoon Low, Janna Ong Abdullah, Chien, Yeong Wee, Rogayah Sekeli, Tan, Chun-Keat, Loh, Jiun-Yan, Lai, Kok-Song
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13048/ http://journalarticle.ukm.my/13048/ http://journalarticle.ukm.my/13048/1/02%20Lee-Yoon%20Low.pdf

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spelling	ukm-130482019-06-20T15:22:40Z http://journalarticle.ukm.my/13048/ Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice Lee, Yoon Low Janna Ong Abdullah, Chien, Yeong Wee Rogayah Sekeli, Tan, Chun-Keat Loh, Jiun-Yan Lai, Kok-Song In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood industry, commonly used as a plant growth enhancer. Seed derived calli were proliferated on Murashige and Skoog (MS) medium supplemented with different ion-chelated LS (calcium LS: CaLS and sodium LS: NaLS) at 50, 100, 150, and 200 mg/L. MS supplemented with 100 mg/L CaLS significantly increased the callus proliferation rate and adventitious root formation. In shoot induction study, both LSs did not enhance the shoot induction efficiency as compared to the control. However, the formation of albino shoot increased in MS fortified with 100 mg/L CaLS. Further chlorophyll and molecular analyses showed that, albino shoots induced from 100 mg/L CaLS had severe reduction in total chlorophyll content and expression of both chlorophyll-associated genes, chlorophyll a/b-binding protein 1 (OsCAB1R) and young seedling albino (OsYSA). Taken together, LS improves callus proliferation rate and modulate different physiological responses during plant growth of recalcitrant indica rice. Penerbit Universiti Kebangsaan Malaysia 2019-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13048/1/02%20Lee-Yoon%20Low.pdf Lee, Yoon Low and Janna Ong Abdullah, and Chien, Yeong Wee and Rogayah Sekeli, and Tan, Chun-Keat and Loh, Jiun-Yan and Lai, Kok-Song (2019) Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice. Sains Malaysiana, 48 (1). pp. 7-13. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In vitro culture of recalcitrant indica rice cultivar through intervening callus is difficult due to long regeneration period. Therefore, this study was undertaken to evaluate the growth promoting effects of lignosulfonate (LS) on callus proliferation and shoot induction of Malaysian recalcitrant indica rice cv. MR219. LS is a by-product of wood industry, commonly used as a plant growth enhancer. Seed derived calli were proliferated on Murashige and Skoog (MS) medium supplemented with different ion-chelated LS (calcium LS: CaLS and sodium LS: NaLS) at 50, 100, 150, and 200 mg/L. MS supplemented with 100 mg/L CaLS significantly increased the callus proliferation rate and adventitious root formation. In shoot induction study, both LSs did not enhance the shoot induction efficiency as compared to the control. However, the formation of albino shoot increased in MS fortified with 100 mg/L CaLS. Further chlorophyll and molecular analyses showed that, albino shoots induced from 100 mg/L CaLS had severe reduction in total chlorophyll content and expression of both chlorophyll-associated genes, chlorophyll a/b-binding protein 1 (OsCAB1R) and young seedling albino (OsYSA). Taken together, LS improves callus proliferation rate and modulate different physiological responses during plant growth of recalcitrant indica rice.
format	Article
author	Lee, Yoon Low Janna Ong Abdullah, Chien, Yeong Wee Rogayah Sekeli, Tan, Chun-Keat Loh, Jiun-Yan Lai, Kok-Song
spellingShingle	Lee, Yoon Low Janna Ong Abdullah, Chien, Yeong Wee Rogayah Sekeli, Tan, Chun-Keat Loh, Jiun-Yan Lai, Kok-Song Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
author_facet	Lee, Yoon Low Janna Ong Abdullah, Chien, Yeong Wee Rogayah Sekeli, Tan, Chun-Keat Loh, Jiun-Yan Lai, Kok-Song
author_sort	Lee, Yoon Low
title	Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
title_short	Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
title_full	Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
title_fullStr	Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
title_full_unstemmed	Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice
title_sort	effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant indica rice
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13048/ http://journalarticle.ukm.my/13048/ http://journalarticle.ukm.my/13048/1/02%20Lee-Yoon%20Low.pdf
first_indexed	2023-09-18T20:03:58Z
last_indexed	2023-09-18T20:03:58Z
_version_	1777407034784743424

Effects of lignosulfonates on callus proliferation and shoot induction of recalcitrant Indica rice

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