The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta

Hibiscus sabdariffa Linn. or also known as roselle which is rich in polyphenols, has been demonstrated to cause lowering of blood pressure in animal and clinical settings. However its exact mechanism of action particularly from polyphenolic compounds is not clearly understood. Therefore, we aimed to...

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Main Authors:	Ahmad Rohi Ghazali, Asmariah Ahmad, Lim, Yi Cheng, Shafreena Shaukat Ali, Satirah Zainalabidin
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12940/ http://journalarticle.ukm.my/12940/ http://journalarticle.ukm.my/12940/1/24002-76978-1-PB.pdf

id	ukm-12940
recordtype	eprints
spelling	ukm-129402019-05-17T14:37:55Z http://journalarticle.ukm.my/12940/ The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta Ahmad Rohi Ghazali, Asmariah Ahmad, Lim, Yi Cheng Shafreena Shaukat Ali, Satirah Zainalabidin, Hibiscus sabdariffa Linn. or also known as roselle which is rich in polyphenols, has been demonstrated to cause lowering of blood pressure in animal and clinical settings. However its exact mechanism of action particularly from polyphenolic compounds is not clearly understood. Therefore, we aimed to determine the effects of H. sabdariffa polyphenol extract (HPE) towards vascular reactivity and its mechanism of action. The HPE was studied on isolated thoracic aortic rings from normal Sprague-Dawley rats, suspended in a 15-ml organ chambers containing Krebs-Henseleit solution. The changes in tension were recorded by isometric transducer connected to data acquisition. HPE relaxed the contraction induced by phenylephrine (PE, 1 μM) in similar pattern for both endothelium-intact and endothelium denuded aortic rings in dose-dependent manner 0.1 ~ 0.9 mg/ml. The pretreatment with atropine (1 μM), a competitive muscarinic antagonist, and propranolol (1 μM), a non-selective beta- blocker did not alter HPE vasorelaxation response. In addition, HPE did not inhibit the contraction induced by extracellular Ca2+ precontracted by PE (1 μM) or KCl (60 mM), in Ca2+ -free solution, suggesting that the relaxation effect of HPE was not via inhibition of calcium channels. In conclusion, HPE demonstrated vasorelaxation effects on rat thoracic aorta although the underlying mechanism is still unknown. The vasorelaxation effect could be via angiotensin type 1 receptor inhibition in the vascular smooth muscle cells or the activation of hyperpolarizing K+ channel. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12940/1/24002-76978-1-PB.pdf Ahmad Rohi Ghazali, and Asmariah Ahmad, and Lim, Yi Cheng and Shafreena Shaukat Ali, and Satirah Zainalabidin, (2018) The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta. Jurnal Sains Kesihatan Malaysia, 16 (SI). pp. 47-53. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1072
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Hibiscus sabdariffa Linn. or also known as roselle which is rich in polyphenols, has been demonstrated to cause lowering of blood pressure in animal and clinical settings. However its exact mechanism of action particularly from polyphenolic compounds is not clearly understood. Therefore, we aimed to determine the effects of H. sabdariffa polyphenol extract (HPE) towards vascular reactivity and its mechanism of action. The HPE was studied on isolated thoracic aortic rings from normal Sprague-Dawley rats, suspended in a 15-ml organ chambers containing Krebs-Henseleit solution. The changes in tension were recorded by isometric transducer connected to data acquisition. HPE relaxed the contraction induced by phenylephrine (PE, 1 μM) in similar pattern for both endothelium-intact and endothelium denuded aortic rings in dose-dependent manner 0.1 ~ 0.9 mg/ml. The pretreatment with atropine (1 μM), a competitive muscarinic antagonist, and propranolol (1 μM), a non-selective beta- blocker did not alter HPE vasorelaxation response. In addition, HPE did not inhibit the contraction induced by extracellular Ca2+ precontracted by PE (1 μM) or KCl (60 mM), in Ca2+ -free solution, suggesting that the relaxation effect of HPE was not via inhibition of calcium channels. In conclusion, HPE demonstrated vasorelaxation effects on rat thoracic aorta although the underlying mechanism is still unknown. The vasorelaxation effect could be via angiotensin type 1 receptor inhibition in the vascular smooth muscle cells or the activation of hyperpolarizing K+ channel.
format	Article
author	Ahmad Rohi Ghazali, Asmariah Ahmad, Lim, Yi Cheng Shafreena Shaukat Ali, Satirah Zainalabidin,
spellingShingle	Ahmad Rohi Ghazali, Asmariah Ahmad, Lim, Yi Cheng Shafreena Shaukat Ali, Satirah Zainalabidin, The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
author_facet	Ahmad Rohi Ghazali, Asmariah Ahmad, Lim, Yi Cheng Shafreena Shaukat Ali, Satirah Zainalabidin,
author_sort	Ahmad Rohi Ghazali,
title	The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
title_short	The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
title_full	The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
title_fullStr	The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
title_full_unstemmed	The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta
title_sort	vasorelaxant effect of hibiscus sabdariffa linn. polyphenol-rich extract (hpe) on rat’s isolated aorta
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12940/ http://journalarticle.ukm.my/12940/ http://journalarticle.ukm.my/12940/1/24002-76978-1-PB.pdf
first_indexed	2023-09-18T20:03:44Z
last_indexed	2023-09-18T20:03:44Z
_version_	1777407019624431616

The vasorelaxant effect of Hibiscus sabdariffa linn. Polyphenol-rich extract (HPE) on rat’s isolated aorta

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