Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line

Drug Metabolizing Enzyme (DME) has been a target of natural chemopreventive agents to inhibit, retard and reverse the process of carcinogenesis. Pterostilbene, an analog to resveratrol has been reported to possess various pharmacological benefits including chemoprevention. In our study, benzo[a]pyre...

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Main Authors:	Ahmad Rohi Ghazali, Lee, Wee Xian, Cheng, Xiang Yi, Asmariah Ahmad, Tava Shelan Nagapan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12937/ http://journalarticle.ukm.my/12937/ http://journalarticle.ukm.my/12937/1/23986-76975-1-PB.pdf

id	ukm-12937
recordtype	eprints
spelling	ukm-129372019-05-13T03:19:06Z http://journalarticle.ukm.my/12937/ Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line Ahmad Rohi Ghazali, Lee, Wee Xian Cheng, Xiang Yi Asmariah Ahmad, Tava Shelan Nagapan, Drug Metabolizing Enzyme (DME) has been a target of natural chemopreventive agents to inhibit, retard and reverse the process of carcinogenesis. Pterostilbene, an analog to resveratrol has been reported to possess various pharmacological benefits including chemoprevention. In our study, benzo[a]pyrene-induced HT-29 colorectal cell line was used as the DME model. The activity of phase I enzyme CYP1A as determined by the 7-ethoxyresorufin O-deethylation (EROD) assay was found to be inhibited significantly by pterostilbene at 50 μM, 75 μM and 100 μM (p ≤ 0.01, p ≤ 0.05, p ≤ 0.01 respectively) compared to the benzo[a]pyrene treated group. Meanwhile, pterostilbene induced glutathione-S-transferase (GST) activity significantly (p ≤ 0.01) at 50 μM as compared to the untreated. In addition, However, the protein expression of CYP1A1 and GST in pterostilbene treated group was not significantly affected compared to untreated. On the other hand, pterostilbene at 25 and 75 μM were able to increase the protein expression of transcription factor Nrf2 significantly (p ≤ 0.01). Results indicated that pterostilbene could reduce metabolic activation of procarcinogens and increase the detoxification process which can be potentially developed as chemopreventive agent. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12937/1/23986-76975-1-PB.pdf Ahmad Rohi Ghazali, and Lee, Wee Xian and Cheng, Xiang Yi and Asmariah Ahmad, and Tava Shelan Nagapan, (2018) Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line. Jurnal Sains Kesihatan Malaysia, 16 (SI). pp. 27-33. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1072
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Drug Metabolizing Enzyme (DME) has been a target of natural chemopreventive agents to inhibit, retard and reverse the process of carcinogenesis. Pterostilbene, an analog to resveratrol has been reported to possess various pharmacological benefits including chemoprevention. In our study, benzo[a]pyrene-induced HT-29 colorectal cell line was used as the DME model. The activity of phase I enzyme CYP1A as determined by the 7-ethoxyresorufin O-deethylation (EROD) assay was found to be inhibited significantly by pterostilbene at 50 μM, 75 μM and 100 μM (p ≤ 0.01, p ≤ 0.05, p ≤ 0.01 respectively) compared to the benzo[a]pyrene treated group. Meanwhile, pterostilbene induced glutathione-S-transferase (GST) activity significantly (p ≤ 0.01) at 50 μM as compared to the untreated. In addition, However, the protein expression of CYP1A1 and GST in pterostilbene treated group was not significantly affected compared to untreated. On the other hand, pterostilbene at 25 and 75 μM were able to increase the protein expression of transcription factor Nrf2 significantly (p ≤ 0.01). Results indicated that pterostilbene could reduce metabolic activation of procarcinogens and increase the detoxification process which can be potentially developed as chemopreventive agent.
format	Article
author	Ahmad Rohi Ghazali, Lee, Wee Xian Cheng, Xiang Yi Asmariah Ahmad, Tava Shelan Nagapan,
spellingShingle	Ahmad Rohi Ghazali, Lee, Wee Xian Cheng, Xiang Yi Asmariah Ahmad, Tava Shelan Nagapan, Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
author_facet	Ahmad Rohi Ghazali, Lee, Wee Xian Cheng, Xiang Yi Asmariah Ahmad, Tava Shelan Nagapan,
author_sort	Ahmad Rohi Ghazali,
title	Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
title_short	Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
title_full	Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
title_fullStr	Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
title_full_unstemmed	Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
title_sort	effects of pterostilbene on activities and protein expression of cytochrome p450 1a1 (cyp1a1) and glutathione s-transferase (gst) in benzo[a]pyrene-induced ht-29 colorectal cancer cell line
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12937/ http://journalarticle.ukm.my/12937/ http://journalarticle.ukm.my/12937/1/23986-76975-1-PB.pdf
first_indexed	2023-09-18T20:03:43Z
last_indexed	2023-09-18T20:03:43Z
_version_	1777407019175641088

Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line

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