Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)

Mutagenic and antimutagenic activities of lactic acid bacteria (LAB) Lactobacillus plantarum isolated from the local fermented durian (tempoyak) was determined by Ames test (Salmonella/microsome mutagenicity assay). Our study also involved pre-incubation assay against Salmonella typhimurium TA 98 an...

Full description

Bibliographic Details
Main Authors:	Asmariah Ahmad, Safura Salik, Yap, Wei Boon, Noorhisham Tan Kofli, Ahmad Rohi Ghazali
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12936/ http://journalarticle.ukm.my/12936/ http://journalarticle.ukm.my/12936/1/23983-76974-1-PB.pdf

id	ukm-12936
recordtype	eprints
spelling	ukm-129362019-05-17T14:33:36Z http://journalarticle.ukm.my/12936/ Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak) Asmariah Ahmad, Safura Salik, Yap, Wei Boon Noorhisham Tan Kofli, Ahmad Rohi Ghazali, Mutagenic and antimutagenic activities of lactic acid bacteria (LAB) Lactobacillus plantarum isolated from the local fermented durian (tempoyak) was determined by Ames test (Salmonella/microsome mutagenicity assay). Our study also involved pre-incubation assay against Salmonella typhimurium TA 98 and TA 100 bacterial strain in the presence and absence of metabolic activator S9 system. It was found that the L. plantarum showed no mutagenic activity on both S. typhimurium strain TA 98 and TA 100 in the presence and absence of metabolic activator. Significant antimutagenic activity (p < 0.05) was observed in both cell-free supernatant and bacterial cell suspension of L. plantarum as compared to the mutagenicity induced by 2-Aminoanthracene in the presence of metabolic activator. Meanwhile, in the absence of metabolic activator, only the bacterial cells of L. plantarum showed antimutagenicity acitivity against Sodium Azide and 2-Nitrofluorene. In conclusion, L. plantarum could play a vital role as chemopreventive agent by binding to mutagens and suppressing mutagenesis. Thus, L. plantarum could be consider as a good candidate for functional food development as a supplement product to prevent development of colon cancer. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12936/1/23983-76974-1-PB.pdf Asmariah Ahmad, and Safura Salik, and Yap, Wei Boon and Noorhisham Tan Kofli, and Ahmad Rohi Ghazali, (2018) Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak). Jurnal Sains Kesihatan Malaysia, 16 (SI). pp. 23-26. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1072
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Mutagenic and antimutagenic activities of lactic acid bacteria (LAB) Lactobacillus plantarum isolated from the local fermented durian (tempoyak) was determined by Ames test (Salmonella/microsome mutagenicity assay). Our study also involved pre-incubation assay against Salmonella typhimurium TA 98 and TA 100 bacterial strain in the presence and absence of metabolic activator S9 system. It was found that the L. plantarum showed no mutagenic activity on both S. typhimurium strain TA 98 and TA 100 in the presence and absence of metabolic activator. Significant antimutagenic activity (p < 0.05) was observed in both cell-free supernatant and bacterial cell suspension of L. plantarum as compared to the mutagenicity induced by 2-Aminoanthracene in the presence of metabolic activator. Meanwhile, in the absence of metabolic activator, only the bacterial cells of L. plantarum showed antimutagenicity acitivity against Sodium Azide and 2-Nitrofluorene. In conclusion, L. plantarum could play a vital role as chemopreventive agent by binding to mutagens and suppressing mutagenesis. Thus, L. plantarum could be consider as a good candidate for functional food development as a supplement product to prevent development of colon cancer.
format	Article
author	Asmariah Ahmad, Safura Salik, Yap, Wei Boon Noorhisham Tan Kofli, Ahmad Rohi Ghazali,
spellingShingle	Asmariah Ahmad, Safura Salik, Yap, Wei Boon Noorhisham Tan Kofli, Ahmad Rohi Ghazali, Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
author_facet	Asmariah Ahmad, Safura Salik, Yap, Wei Boon Noorhisham Tan Kofli, Ahmad Rohi Ghazali,
author_sort	Asmariah Ahmad,
title	Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
title_short	Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
title_full	Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
title_fullStr	Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
title_full_unstemmed	Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)
title_sort	mutagenicity and antimutagenic activities of lactic acid bacteria (lab) isolated from fermented durian (tempoyak)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12936/ http://journalarticle.ukm.my/12936/ http://journalarticle.ukm.my/12936/1/23983-76974-1-PB.pdf
first_indexed	2023-09-18T20:03:43Z
last_indexed	2023-09-18T20:03:43Z
_version_	1777407019028840448

Mutagenicity and antimutagenic activities of lactic acid bacteria (LAB) isolated from fermented durian (tempoyak)

Similar Items