Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture

Sesquiterpenes are a three-isoprene unit compounds which belong to terpenoid family of secondary metabolites. These volatile compounds are one of the major constituents of essential oils in plants and plays major roles in plant signaling of defense mechanism. The effects of methyl jasmonate (MeJA) c...

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Main Authors:	Piriyatasni Sellapan, Emelda Rosseleena Rohani, Normah Mohd Noor
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12927/ http://journalarticle.ukm.my/12927/ http://journalarticle.ukm.my/12927/1/15%20Piriyatasni%20Sellapan.pdf

id	ukm-12927
recordtype	eprints
spelling	ukm-129272019-05-15T11:07:05Z http://journalarticle.ukm.my/12927/ Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture Piriyatasni Sellapan, Emelda Rosseleena Rohani, Normah Mohd Noor, Sesquiterpenes are a three-isoprene unit compounds which belong to terpenoid family of secondary metabolites. These volatile compounds are one of the major constituents of essential oils in plants and plays major roles in plant signaling of defense mechanism. The effects of methyl jasmonate (MeJA) concentrations (100 and 200 μM) on the production of sesquiterpene compounds after incubation period for 1, 3, and 6 days were investigated in Persicaria minor cell suspension culture. Headspace Solid-Phase Microextraction (HS-SPME) method was used to absorb volatile compounds from suspension cells and liquid medium. They were then analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) to identify sesquiterpene compounds. Among the 15 sesquiterpene compounds identified, α- muurolene was found in significantly higher concentration in all MeJA treated cultures. The results showed that α-muurolene was detected in the suspension cells at the highest peak area of 14.17% at 100 μM MeJA treated cultures with 3-day incubation. Analysis of liquid medium of the treatments identified secretion of α-muurolene into the culture medium, with total peak area of 0.72%. These results showed that sesquiterpene production in MeJA induced P. minor suspension culture depended on the MeJA concentration and also culture incubation period. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12927/1/15%20Piriyatasni%20Sellapan.pdf Piriyatasni Sellapan, and Emelda Rosseleena Rohani, and Normah Mohd Noor, (2018) Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture. Sains Malaysiana, 47 (12). pp. 3051-3059. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Sesquiterpenes are a three-isoprene unit compounds which belong to terpenoid family of secondary metabolites. These volatile compounds are one of the major constituents of essential oils in plants and plays major roles in plant signaling of defense mechanism. The effects of methyl jasmonate (MeJA) concentrations (100 and 200 μM) on the production of sesquiterpene compounds after incubation period for 1, 3, and 6 days were investigated in Persicaria minor cell suspension culture. Headspace Solid-Phase Microextraction (HS-SPME) method was used to absorb volatile compounds from suspension cells and liquid medium. They were then analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) to identify sesquiterpene compounds. Among the 15 sesquiterpene compounds identified, α- muurolene was found in significantly higher concentration in all MeJA treated cultures. The results showed that α-muurolene was detected in the suspension cells at the highest peak area of 14.17% at 100 μM MeJA treated cultures with 3-day incubation. Analysis of liquid medium of the treatments identified secretion of α-muurolene into the culture medium, with total peak area of 0.72%. These results showed that sesquiterpene production in MeJA induced P. minor suspension culture depended on the MeJA concentration and also culture incubation period.
format	Article
author	Piriyatasni Sellapan, Emelda Rosseleena Rohani, Normah Mohd Noor,
spellingShingle	Piriyatasni Sellapan, Emelda Rosseleena Rohani, Normah Mohd Noor, Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
author_facet	Piriyatasni Sellapan, Emelda Rosseleena Rohani, Normah Mohd Noor,
author_sort	Piriyatasni Sellapan,
title	Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
title_short	Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
title_full	Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
title_fullStr	Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
title_full_unstemmed	Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture
title_sort	sesquiterpene production in methyl jasmonate-induced persicaria minor cell suspension culture
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12927/ http://journalarticle.ukm.my/12927/ http://journalarticle.ukm.my/12927/1/15%20Piriyatasni%20Sellapan.pdf
first_indexed	2023-09-18T20:03:42Z
last_indexed	2023-09-18T20:03:42Z
_version_	1777407017726509056

Sesquiterpene production in methyl jasmonate-induced Persicaria minor cell suspension culture

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