Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products

F-box proteins containing variable C-terminal domains make an essential part of SKP1-Cullin-Ring box-F box (SCF) complex. SCF complex catalyzes the final step to link the ubiquitin tag with the target protein, destined for degradation, through F-box protein that confer overall substrate specificity...

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Main Authors:	ul-Hassan, Muhammad Naeem, Zamri Zainal, Nur Athirah Abd Hamid, Muhammad Sajad, Ismanizan Ismail
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12921/ http://journalarticle.ukm.my/12921/ http://journalarticle.ukm.my/12921/1/09%20Muhammad%20Naeem-Ul-Hassan.pdf

id	ukm-12921
recordtype	eprints
spelling	ukm-129212019-05-15T11:02:21Z http://journalarticle.ukm.my/12921/ Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products ul-Hassan, Muhammad Naeem Zamri Zainal, Nur Athirah Abd Hamid, Muhammad Sajad, Ismanizan Ismail, F-box proteins containing variable C-terminal domains make an essential part of SKP1-Cullin-Ring box-F box (SCF) complex. SCF complex catalyzes the final step to link the ubiquitin tag with the target protein, destined for degradation, through F-box protein that confer overall substrate specificity to the complex. In this study, we analyzed the role of At2g02870, a Kelch containing F-box protein from Arabidopsis thaliana, by using reverse genetics strategy. At2g02870 loss of function mutant lines (at2g02870) were analyzed and compared with wild type plants for the expression of genes and products of hydroperoxide lyase (HPL) branch of oxylipin pathway. We found that the at2g02870 plants have enhanced expression of HPL pathway genes and produce more green leaf volatiles (GLV) than the wild type plants. Our results suggested that the gene is involved in the regulation of HPL pathway, possibly through the degradation of enzymes or/and the regulatory factors of the pathway. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12921/1/09%20Muhammad%20Naeem-Ul-Hassan.pdf ul-Hassan, Muhammad Naeem and Zamri Zainal, and Nur Athirah Abd Hamid, and Muhammad Sajad, and Ismanizan Ismail, (2018) Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products. Sains Malaysiana, 47 (12). pp. 3003-3008. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	F-box proteins containing variable C-terminal domains make an essential part of SKP1-Cullin-Ring box-F box (SCF) complex. SCF complex catalyzes the final step to link the ubiquitin tag with the target protein, destined for degradation, through F-box protein that confer overall substrate specificity to the complex. In this study, we analyzed the role of At2g02870, a Kelch containing F-box protein from Arabidopsis thaliana, by using reverse genetics strategy. At2g02870 loss of function mutant lines (at2g02870) were analyzed and compared with wild type plants for the expression of genes and products of hydroperoxide lyase (HPL) branch of oxylipin pathway. We found that the at2g02870 plants have enhanced expression of HPL pathway genes and produce more green leaf volatiles (GLV) than the wild type plants. Our results suggested that the gene is involved in the regulation of HPL pathway, possibly through the degradation of enzymes or/and the regulatory factors of the pathway.
format	Article
author	ul-Hassan, Muhammad Naeem Zamri Zainal, Nur Athirah Abd Hamid, Muhammad Sajad, Ismanizan Ismail,
spellingShingle	ul-Hassan, Muhammad Naeem Zamri Zainal, Nur Athirah Abd Hamid, Muhammad Sajad, Ismanizan Ismail, Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
author_facet	ul-Hassan, Muhammad Naeem Zamri Zainal, Nur Athirah Abd Hamid, Muhammad Sajad, Ismanizan Ismail,
author_sort	ul-Hassan, Muhammad Naeem
title	Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
title_short	Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
title_full	Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
title_fullStr	Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
title_full_unstemmed	Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
title_sort	arabidopsis at2g02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12921/ http://journalarticle.ukm.my/12921/ http://journalarticle.ukm.my/12921/1/09%20Muhammad%20Naeem-Ul-Hassan.pdf
first_indexed	2023-09-18T20:03:41Z
last_indexed	2023-09-18T20:03:41Z
_version_	1777407016855142400

Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products

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