Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)

Plutella xylostella (L.) (Lepidoptera: Plutellidae), the major insect pest of cruciferous crops worldwide shows significant resistance to almost all classes of insecticides. In order to effectively prevent and manage the insecticidal resistance, it is crucial to understand the physiological adaptati...

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Main Authors:	Maizom Hassan, Norazila Yusoff, Wan Mohd Aizat, Nurul Wahida Othman, Idris Abd Ghani
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12918/ http://journalarticle.ukm.my/12918/ http://journalarticle.ukm.my/12918/1/06%20Maizom%20Hassan.pdf

id	ukm-12918
recordtype	eprints
spelling	ukm-129182019-05-15T10:59:44Z http://journalarticle.ukm.my/12918/ Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae) Maizom Hassan, Norazila Yusoff, Wan Mohd Aizat, Nurul Wahida Othman, Idris Abd Ghani, Plutella xylostella (L.) (Lepidoptera: Plutellidae), the major insect pest of cruciferous crops worldwide shows significant resistance to almost all classes of insecticides. In order to effectively prevent and manage the insecticidal resistance, it is crucial to understand the physiological adaptation of insects against insecticides. Identification of insect protein that interacting with insecticides and characterization of their modification in resistant strains can be done by using differential proteomics approach. This study focuses on optimizing a sensitive and rapid method for the extraction of high quality protein of both larva and adult tissues of P. xylostella to be used in two-dimensional gel electrophoresis. Five extraction methods were evaluated for protein concentration, yields and resolving patterns of one-dimensional and two-dimensional electrophoresis. The results showed that trichloroacetic acid/acetone extraction methods with two different concentrations of 2-mercaptoethanol produced the highest protein concentration and yield for both adult and larva tissues, respectively. Meanwhile, trichloroacetic acid/acetone with dithiothreitol extraction method gave better separation of spots and intensity for both larva and adult tissues compared to other methods tested. As such, we concluded that trichloroacetic acid/acetone with dithiothreitol successfully yielded high total protein concentration and good separation of two-dimensional electrophoresis gel spots in both adult and larva P. xylostella. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12918/1/06%20Maizom%20Hassan.pdf Maizom Hassan, and Norazila Yusoff, and Wan Mohd Aizat, and Nurul Wahida Othman, and Idris Abd Ghani, (2018) Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae). Sains Malaysiana, 47 (12). pp. 2975-2983. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Plutella xylostella (L.) (Lepidoptera: Plutellidae), the major insect pest of cruciferous crops worldwide shows significant resistance to almost all classes of insecticides. In order to effectively prevent and manage the insecticidal resistance, it is crucial to understand the physiological adaptation of insects against insecticides. Identification of insect protein that interacting with insecticides and characterization of their modification in resistant strains can be done by using differential proteomics approach. This study focuses on optimizing a sensitive and rapid method for the extraction of high quality protein of both larva and adult tissues of P. xylostella to be used in two-dimensional gel electrophoresis. Five extraction methods were evaluated for protein concentration, yields and resolving patterns of one-dimensional and two-dimensional electrophoresis. The results showed that trichloroacetic acid/acetone extraction methods with two different concentrations of 2-mercaptoethanol produced the highest protein concentration and yield for both adult and larva tissues, respectively. Meanwhile, trichloroacetic acid/acetone with dithiothreitol extraction method gave better separation of spots and intensity for both larva and adult tissues compared to other methods tested. As such, we concluded that trichloroacetic acid/acetone with dithiothreitol successfully yielded high total protein concentration and good separation of two-dimensional electrophoresis gel spots in both adult and larva P. xylostella.
format	Article
author	Maizom Hassan, Norazila Yusoff, Wan Mohd Aizat, Nurul Wahida Othman, Idris Abd Ghani,
spellingShingle	Maizom Hassan, Norazila Yusoff, Wan Mohd Aizat, Nurul Wahida Othman, Idris Abd Ghani, Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
author_facet	Maizom Hassan, Norazila Yusoff, Wan Mohd Aizat, Nurul Wahida Othman, Idris Abd Ghani,
author_sort	Maizom Hassan,
title	Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
title_short	Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
title_full	Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
title_fullStr	Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
title_full_unstemmed	Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)
title_sort	optimization method for proteomic analysis of the larva and adult tissues of plutella xylostella (l.) (lepidoptera: plutellidae)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12918/ http://journalarticle.ukm.my/12918/ http://journalarticle.ukm.my/12918/1/06%20Maizom%20Hassan.pdf
first_indexed	2023-09-18T20:03:41Z
last_indexed	2023-09-18T20:03:41Z
_version_	1777407016410546176

Optimization method for proteomic analysis of the larva and adult tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)

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