Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu

Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic...

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Main Authors:	Chai, Jun Meng, Manogiri Sadasivam, Sevakumaran Vigneswari
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/1/48_01_28.pdf

id	ukm-12797
recordtype	eprints
spelling	ukm-127972019-04-16T09:11:55Z http://journalarticle.ukm.my/12797/ Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years. Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp. KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria isolated can be tailored for various biomedical applications. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12797/1/48_01_28.pdf Chai, Jun Meng and Manogiri Sadasivam, and Sevakumaran Vigneswari, (2019) Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu. Malaysian Applied Biology, 48 (1). pp. 199-206. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years. Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp. KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria isolated can be tailored for various biomedical applications.
format	Article
author	Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari,
spellingShingle	Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
author_facet	Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari,
author_sort	Chai, Jun Meng
title	Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_short	Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_full	Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_fullStr	Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_full_unstemmed	Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_sort	isolation and identification of p(3hb-co-4hb) producing bacteria from various locations in kuala terengganu
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/1/48_01_28.pdf
first_indexed	2023-09-18T20:03:24Z
last_indexed	2023-09-18T20:03:24Z
_version_	1777406998713729024

Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu

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