Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/1/48_01_28.pdf |
| Summary: | Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in
the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems
with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production
of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years.
Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought
after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this
study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of
lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates
were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed
as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain
were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp.
KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria
isolated can be tailored for various biomedical applications. |
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