Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu

Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are...

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Main Authors:	Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12793/ http://journalarticle.ukm.my/12793/ http://journalarticle.ukm.my/12793/1/48_01_24.pdf

id	ukm-12793
recordtype	eprints
spelling	ukm-127932019-04-16T08:59:18Z http://journalarticle.ukm.my/12793/ Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel, Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are recycled through the “microbial loop”. Thus, understanding the connection between the diversity composition and functional role of aquatic microbial community is crucial for proper lake management. This study aims to determine the diversity and functions of microbial assemblages in Tasik Kenyir by means of shotgun metagenomics analysis. Briefly, water samples were collected from pristine and disturbed areas. Metagenome DNA were then extracted directly and subjected to clone-independent sequencing. Data sequences of all samples were analyzed and functional annotated using bioinformatics software MEGAN6. Analysis showed up to 41 phyla that had been detected from the water samples with the presence of dominant bacterial populations of more than 90% in all samples. Proteobacteria was the most dominant phylum, representing more than 70% of the microbiome in all samples. Other taxa such as Bacteriodetes, Terrabacteria group, Verrucomicrobia, Planctomycetes and Chloroflexi were also found as part of the microbial communities. The first sample from the disturbed area, TKSA1 had 3% of total contigs read assigned to genera Pseudomonas while the other samples appeared to be more homogeneous. The lake also appeared to contain a mixture of autotrophs and heterotrophs capable of performing main biogeochemical cycles. Findings of the present study has provided us valuable information on the microbial diversity structures and their functions in the nutrient processing pathways that occurred in the Tasik Kenyir environment and thus sheds light on the importance of freshwater microbial communities for ecosystem and human health. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12793/1/48_01_24.pdf Mohd Ezhar Mohd Noor, and Sharifah Noor Emilia Syed Jamil Fadaak, and Mohd Noor Mat Isa, and Mohd Faizal Abu Bakar, and Muhd Danish-Daniel, (2019) Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu. Malaysian Applied Biology, 48 (1). pp. 169-175. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are recycled through the “microbial loop”. Thus, understanding the connection between the diversity composition and functional role of aquatic microbial community is crucial for proper lake management. This study aims to determine the diversity and functions of microbial assemblages in Tasik Kenyir by means of shotgun metagenomics analysis. Briefly, water samples were collected from pristine and disturbed areas. Metagenome DNA were then extracted directly and subjected to clone-independent sequencing. Data sequences of all samples were analyzed and functional annotated using bioinformatics software MEGAN6. Analysis showed up to 41 phyla that had been detected from the water samples with the presence of dominant bacterial populations of more than 90% in all samples. Proteobacteria was the most dominant phylum, representing more than 70% of the microbiome in all samples. Other taxa such as Bacteriodetes, Terrabacteria group, Verrucomicrobia, Planctomycetes and Chloroflexi were also found as part of the microbial communities. The first sample from the disturbed area, TKSA1 had 3% of total contigs read assigned to genera Pseudomonas while the other samples appeared to be more homogeneous. The lake also appeared to contain a mixture of autotrophs and heterotrophs capable of performing main biogeochemical cycles. Findings of the present study has provided us valuable information on the microbial diversity structures and their functions in the nutrient processing pathways that occurred in the Tasik Kenyir environment and thus sheds light on the importance of freshwater microbial communities for ecosystem and human health.
format	Article
author	Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel,
spellingShingle	Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel, Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
author_facet	Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel,
author_sort	Mohd Ezhar Mohd Noor,
title	Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_short	Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_full	Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_fullStr	Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_full_unstemmed	Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_sort	biodiversity and functional metagenomic profiling of microbial communities in tasik kenyir, terengganu
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12793/ http://journalarticle.ukm.my/12793/ http://journalarticle.ukm.my/12793/1/48_01_24.pdf
first_indexed	2023-09-18T20:03:23Z
last_indexed	2023-09-18T20:03:23Z
_version_	1777406998102409216

Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu

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