Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu

This research was carried out to examine the presence of protozoa that inhabit on the gills of hybrid grouper. This phylum can act as ecto and endoparasites in fish and in turn are responsible for many diseases. Observation on cultured samples by using swabbing techniques on the gill surface indic...

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Main Authors:	Fatimah Hashim, Malinna Jusoh, Nik Nur Izzati Nik Rohin, Nur Al Syifaa Hassan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/12792/ http://journalarticle.ukm.my/12792/ http://journalarticle.ukm.my/12792/1/48_01_23.pdf

id	ukm-12792
recordtype	eprints
spelling	ukm-127922019-04-16T09:14:21Z http://journalarticle.ukm.my/12792/ Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu Fatimah Hashim, Malinna Jusoh, Nik Nur Izzati Nik Rohin, Nur Al Syifaa Hassan, This research was carried out to examine the presence of protozoa that inhabit on the gills of hybrid grouper. This phylum can act as ecto and endoparasites in fish and in turn are responsible for many diseases. Observation on cultured samples by using swabbing techniques on the gill surface indicated the presence of ciliated protozoan. The morphological features of the vegetative stage ciliates were kidney shaped and highly motile with 49.12 μm in length, whereas the resting stage of the ciliates was round shape with 38.42 μm in length as shown by light microscopy. Observation on the cell surface by scanning electron microscopy indicated unfamiliar silverline system for ciliates and the resting cyst surface structure was mildly wrinkled. Further specific species identification was verified by conducting Polymerase Chain Reaction (PCR). The ciliate gene in the 18S SSU ribosomal RNA isolated was very similar (99% sequence identity) to Colpoda steinii DNA sequence at GenBank under accession number of KJ607912.1 with 100% query cover. Although there were no reports on hybrid grouper mortality in Setiu, due to gills infection by ciliate C. stenii, it was important to obtain information on microorganism inhabitants in hybrid grouper fish gills. It is hoped that this data collected might be an indicator for fish health state for our fish farmers and as a precaution for conserving healthy environment in Setiu for better aquaculture-related production. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12792/1/48_01_23.pdf Fatimah Hashim, and Malinna Jusoh, and Nik Nur Izzati Nik Rohin, and Nur Al Syifaa Hassan, (2019) Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu. Malaysian Applied Biology, 48 (1). pp. 163-168. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This research was carried out to examine the presence of protozoa that inhabit on the gills of hybrid grouper. This phylum can act as ecto and endoparasites in fish and in turn are responsible for many diseases. Observation on cultured samples by using swabbing techniques on the gill surface indicated the presence of ciliated protozoan. The morphological features of the vegetative stage ciliates were kidney shaped and highly motile with 49.12 μm in length, whereas the resting stage of the ciliates was round shape with 38.42 μm in length as shown by light microscopy. Observation on the cell surface by scanning electron microscopy indicated unfamiliar silverline system for ciliates and the resting cyst surface structure was mildly wrinkled. Further specific species identification was verified by conducting Polymerase Chain Reaction (PCR). The ciliate gene in the 18S SSU ribosomal RNA isolated was very similar (99% sequence identity) to Colpoda steinii DNA sequence at GenBank under accession number of KJ607912.1 with 100% query cover. Although there were no reports on hybrid grouper mortality in Setiu, due to gills infection by ciliate C. stenii, it was important to obtain information on microorganism inhabitants in hybrid grouper fish gills. It is hoped that this data collected might be an indicator for fish health state for our fish farmers and as a precaution for conserving healthy environment in Setiu for better aquaculture-related production.
format	Article
author	Fatimah Hashim, Malinna Jusoh, Nik Nur Izzati Nik Rohin, Nur Al Syifaa Hassan,
spellingShingle	Fatimah Hashim, Malinna Jusoh, Nik Nur Izzati Nik Rohin, Nur Al Syifaa Hassan, Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
author_facet	Fatimah Hashim, Malinna Jusoh, Nik Nur Izzati Nik Rohin, Nur Al Syifaa Hassan,
author_sort	Fatimah Hashim,
title	Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
title_short	Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
title_full	Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
title_fullStr	Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
title_full_unstemmed	Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu
title_sort	colpoda steinii associated with hybrid grouper from kg. gong batu setiu, terengganu
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/12792/ http://journalarticle.ukm.my/12792/ http://journalarticle.ukm.my/12792/1/48_01_23.pdf
first_indexed	2023-09-18T20:03:23Z
last_indexed	2023-09-18T20:03:23Z
_version_	1777406997960851456

Colpoda steinii associated with hybrid grouper from Kg. Gong Batu Setiu, Terengganu

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