Pineapple honey inhibits adipocytes proliferation and reduces lipid droplet accumulation in 3T3-L1 adipocytes
Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the effects of pineapple honey on the growth an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/ http://journalarticle.ukm.my/12771/1/48_01_04.pdf |
| Summary: | Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride
levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the
effects of pineapple honey on the growth and lipid accumulation of adipocytes in vitro. Pineapple honey was standardised
according to its total phenolic and flavonoid contents prior to treating on differentiated 3T3-L1 adipocytes. Proliferation of
adipocytes was observed using 3-(4,5 dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) assay while lipid
accumulation and droplet size were determined using oil red O staining and Feret’s diameter. Pineapple honey exhibited
0.0379 ± 0.001 mg/100 mL GAE of total phenolic content and 0.098 ± 0.001 mg catechin/kg of total flavonoid content. It
significantly inhibited adipocytes’ proliferation starting from 6.25% of pineapple honey concentration. In addition, the honey
also significantly reduced lipid droplet size by 33.78% to 70.36% and reduced lipid accumulation compared to the control.
These findings suggest that pineapple honey may affect the storage of lipids in adipocytes. Future investigation involving the
biomarkers of adipogenesis is required to confirm whether the reduction in lipid accumulation is attributed to the effect of
honey on these pathways. |
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