Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey

Heterotrigona itama (Family: Apidae, Tribe: Meliponini, Genus: Trigona) is one of the stingless bee in Meliponiculture in Malaysia. H. itama honey is reported to be a good reservoir for novel lactic acid bacteria (LAB) with probiotic properties. In this study, five Lactobacillus brevis strains (st...

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Main Authors:	Nor Hazwani Hasali, Amir Izzwan Zamri, Mohd Nizam Lani, Aidilla Mubarak
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12763/ http://journalarticle.ukm.my/12763/ http://journalarticle.ukm.my/12763/1/47_06_13.pdf

id	ukm-12763
recordtype	eprints
spelling	ukm-127632019-04-04T11:24:09Z http://journalarticle.ukm.my/12763/ Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey Nor Hazwani Hasali, Amir Izzwan Zamri, Mohd Nizam Lani, Aidilla Mubarak, Heterotrigona itama (Family: Apidae, Tribe: Meliponini, Genus: Trigona) is one of the stingless bee in Meliponiculture in Malaysia. H. itama honey is reported to be a good reservoir for novel lactic acid bacteria (LAB) with probiotic properties. In this study, five Lactobacillus brevis strains (strain Ibr-42, strain 37901, strain ATCC 367, strain NJ42 and strain KLDS) were previously isolated and identified from H. itama honey obtained from local stingless beekeepers in the coastal areas in Kelantan and Terengganu, were evaluated for antibiotic resistance, antibacterial activity, resistance to low pH, tolerance to bile salts and haemolytic activity. The results indicated that all five strains of L. brevis were susceptible to chloramphenicol, ampicillin, erythromycin, and tetramycin, but resistant to kanamycin. In terms of antagonistic activity among L. brevis, it was found antagonistic activity was minimum. For antibacterial activities of these strains against selected foodborne pathogenic bacteria by well diffusion method, L. brevis strain NJ42 exhibited the highest inhibition (24 mm) against Pseudomonas aeruginosa ATCC 15442. All Lactobacillus strains from H. itama honey were able to survive in pH 2 and 0.3% (w/v) bile salts concentration that mimic the conditions in the gastrointestinal system. The inability of L. brevis strains to exhibit β- haemolytic activity showing that haemolysis is not the virulence factor for these strains. These findings proved the isolated L. brevis in H. itama honey could be used as potential probiotic, envisaging its potential as one of functional foods for food industry. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12763/1/47_06_13.pdf Nor Hazwani Hasali, and Amir Izzwan Zamri, and Mohd Nizam Lani, and Aidilla Mubarak, (2018) Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey. Malaysian Applied Biology, 47 (6). pp. 105-112. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Heterotrigona itama (Family: Apidae, Tribe: Meliponini, Genus: Trigona) is one of the stingless bee in Meliponiculture in Malaysia. H. itama honey is reported to be a good reservoir for novel lactic acid bacteria (LAB) with probiotic properties. In this study, five Lactobacillus brevis strains (strain Ibr-42, strain 37901, strain ATCC 367, strain NJ42 and strain KLDS) were previously isolated and identified from H. itama honey obtained from local stingless beekeepers in the coastal areas in Kelantan and Terengganu, were evaluated for antibiotic resistance, antibacterial activity, resistance to low pH, tolerance to bile salts and haemolytic activity. The results indicated that all five strains of L. brevis were susceptible to chloramphenicol, ampicillin, erythromycin, and tetramycin, but resistant to kanamycin. In terms of antagonistic activity among L. brevis, it was found antagonistic activity was minimum. For antibacterial activities of these strains against selected foodborne pathogenic bacteria by well diffusion method, L. brevis strain NJ42 exhibited the highest inhibition (24 mm) against Pseudomonas aeruginosa ATCC 15442. All Lactobacillus strains from H. itama honey were able to survive in pH 2 and 0.3% (w/v) bile salts concentration that mimic the conditions in the gastrointestinal system. The inability of L. brevis strains to exhibit β- haemolytic activity showing that haemolysis is not the virulence factor for these strains. These findings proved the isolated L. brevis in H. itama honey could be used as potential probiotic, envisaging its potential as one of functional foods for food industry.
format	Article
author	Nor Hazwani Hasali, Amir Izzwan Zamri, Mohd Nizam Lani, Aidilla Mubarak,
spellingShingle	Nor Hazwani Hasali, Amir Izzwan Zamri, Mohd Nizam Lani, Aidilla Mubarak, Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
author_facet	Nor Hazwani Hasali, Amir Izzwan Zamri, Mohd Nizam Lani, Aidilla Mubarak,
author_sort	Nor Hazwani Hasali,
title	Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
title_short	Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
title_full	Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
title_fullStr	Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
title_full_unstemmed	Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey
title_sort	antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated lactobacillus brevis strains from heterotrigona itama honey
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12763/ http://journalarticle.ukm.my/12763/ http://journalarticle.ukm.my/12763/1/47_06_13.pdf
first_indexed	2023-09-18T20:03:19Z
last_indexed	2023-09-18T20:03:19Z
_version_	1777406994008768512

Antibiotic susceptibility, antibacterial activity and probiotic characterisation of isolated Lactobacillus brevis strains from Heterotrigona itama honey

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