Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene

Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore,...

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Main Authors:	Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12752/ http://journalarticle.ukm.my/12752/ http://journalarticle.ukm.my/12752/1/47_06_05.pdf

id	ukm-12752
recordtype	eprints
spelling	ukm-127522019-04-04T11:34:32Z http://journalarticle.ukm.my/12752/ Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12752/1/47_06_05.pdf Nazia Abdul Kadar, and Sitti Raehanah Muhamad Shaleh, and Emma Su Ali, (2018) Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene. Malaysian Applied Biology, 47 (6). pp. 41-45. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels.
format	Article
author	Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali,
spellingShingle	Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
author_facet	Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali,
author_sort	Nazia Abdul Kadar,
title	Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
title_short	Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
title_full	Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
title_fullStr	Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
title_full_unstemmed	Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
title_sort	molecular and phylogenetic identification of marine microalgae inferred by 18s rdna gene
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12752/ http://journalarticle.ukm.my/12752/ http://journalarticle.ukm.my/12752/1/47_06_05.pdf
first_indexed	2023-09-18T20:03:18Z
last_indexed	2023-09-18T20:03:18Z
_version_	1777406992853237760

Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene

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