$The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver$

The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver

Glutathione S-transferase isoenzymes (GSTs) catalyze the conjugation reaction between glutathione and electrophilic compounds. GSTs are involved in the detoxification of toxic and carcinogenic compounds, thus protecting the body from toxic injuries. Tocotrienols are part of the vitamin E family and...

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Main Authors:	Ahmed Atia, Nadia Salem AlRawaiq, Azman Abdullah
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12662/ http://journalarticle.ukm.my/12662/ http://journalarticle.ukm.my/12662/1/23%20Ahmed%20Atia.pdf

id	ukm-12662
recordtype	eprints
spelling	ukm-126622019-03-17T11:47:17Z http://journalarticle.ukm.my/12662/ The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver Ahmed Atia, Nadia Salem AlRawaiq, Azman Abdullah, Glutathione S-transferase isoenzymes (GSTs) catalyze the conjugation reaction between glutathione and electrophilic compounds. GSTs are involved in the detoxification of toxic and carcinogenic compounds, thus protecting the body from toxic injuries. Tocotrienols are part of the vitamin E family and is believed to possess potent antioxidant activity. The objective of this study was to determine the effect of increasing doses of tocotrienol rich fraction (TRF) supplementation on liver GSTs gene and protein expression. A total of 30 male ICR white mice were divided into five groups (n=6 for each group) and given treatment for 14 days through oral supplementation. Groups were divided as follows: - three groups administered with TRF at doses of 200, 500 and 1000 mg/kg, respectively, a positive control group administered with 100 mg/kg butylated hydroxyanisole (BHA) and a control group administered with only the vehicle (corn oil). At day 15, the mice were sacrificed and their livers isolated. Total RNA was extracted from the liver and quantitative real-time polymerase chain reaction (qPCR) assays were performed to analyze GSTs gene expression. Total liver protein was also extracted and the protein expression of GSTs was determined by Western blotting. The results showed that TRF oral supplementation caused a significant dose-dependent increase in liver GST isoenzymes gene and protein expression, compared to controls. In conclusion, TRF oral supplementation for 14 days resulted in increased gene and protein expression of GST isoenzymes in mice liver dose-dependently, with the highest expression seen in mice treated with 1000 mg/kg TRF. Penerbit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12662/1/23%20Ahmed%20Atia.pdf Ahmed Atia, and Nadia Salem AlRawaiq, and Azman Abdullah, (2018) The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver. Sains Malaysiana, 47 (11). pp. 2799-2809. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num11_2018/contentsVol47num11_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Glutathione S-transferase isoenzymes (GSTs) catalyze the conjugation reaction between glutathione and electrophilic compounds. GSTs are involved in the detoxification of toxic and carcinogenic compounds, thus protecting the body from toxic injuries. Tocotrienols are part of the vitamin E family and is believed to possess potent antioxidant activity. The objective of this study was to determine the effect of increasing doses of tocotrienol rich fraction (TRF) supplementation on liver GSTs gene and protein expression. A total of 30 male ICR white mice were divided into five groups (n=6 for each group) and given treatment for 14 days through oral supplementation. Groups were divided as follows: - three groups administered with TRF at doses of 200, 500 and 1000 mg/kg, respectively, a positive control group administered with 100 mg/kg butylated hydroxyanisole (BHA) and a control group administered with only the vehicle (corn oil). At day 15, the mice were sacrificed and their livers isolated. Total RNA was extracted from the liver and quantitative real-time polymerase chain reaction (qPCR) assays were performed to analyze GSTs gene expression. Total liver protein was also extracted and the protein expression of GSTs was determined by Western blotting. The results showed that TRF oral supplementation caused a significant dose-dependent increase in liver GST isoenzymes gene and protein expression, compared to controls. In conclusion, TRF oral supplementation for 14 days resulted in increased gene and protein expression of GST isoenzymes in mice liver dose-dependently, with the highest expression seen in mice treated with 1000 mg/kg TRF.
format	Article
author	Ahmed Atia, Nadia Salem AlRawaiq, Azman Abdullah,
spellingShingle	Ahmed Atia, Nadia Salem AlRawaiq, Azman Abdullah, The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
author_facet	Ahmed Atia, Nadia Salem AlRawaiq, Azman Abdullah,
author_sort	Ahmed Atia,
title	The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
title_short	The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
title_full	The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
title_fullStr	The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
title_full_unstemmed	The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
title_sort	effect of tocotrienol-rich fraction on the expression of glutathione s-transferase isoenzymes in mice liver
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12662/ http://journalarticle.ukm.my/12662/ http://journalarticle.ukm.my/12662/1/23%20Ahmed%20Atia.pdf
first_indexed	2023-09-18T20:03:07Z
last_indexed	2023-09-18T20:03:07Z
_version_	1777406980425515008

The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver

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