The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model

A balanced interaction between osteoblast and osteoclast plays a major role in maintaining bone strength and structural integrity. Tocotrienol, a palm derivative with potent antioxidant properties showed an anti-osteoporotic effect but little is known about its mechanism of action. Hence, this resea...

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Main Authors:	Mohamad Fairuz Yahaya, Azizi Zainodin, Reinukaa Pupathy, Ong, Edwin Hock Min, Nurul Husna Abu Bakar, Nor Azimah Zamri, Halim Ismail, Elvy Suhana Mohd Ramli
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12655/ http://journalarticle.ukm.my/12655/ http://journalarticle.ukm.my/12655/1/16%20Mohamad%20Fairuz%20Yahaya.pdf

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spelling	ukm-126552019-03-17T11:25:49Z http://journalarticle.ukm.my/12655/ The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model Mohamad Fairuz Yahaya, Azizi Zainodin, Reinukaa Pupathy, Ong, Edwin Hock Min Nurul Husna Abu Bakar, Nor Azimah Zamri, Halim Ismail, Elvy Suhana Mohd Ramli, A balanced interaction between osteoblast and osteoclast plays a major role in maintaining bone strength and structural integrity. Tocotrienol, a palm derivative with potent antioxidant properties showed an anti-osteoporotic effect but little is known about its mechanism of action. Hence, this research was conducted to determine the effects of palm tocotrienol on the surface osteoblast and osteoclast of the the glucocorticoid-induced osteoporotic bones. The study involved 40 male Sprague-Dawley rats weighing 250-300 g which were randomly divided into an equal number of Baseline, Sham, Adrx+Dexa (adrenalectomized with dexamethasone), Adrx+Dexa+ATF (adrenalectomized with dexamethasone supplemented with α-tocopherol) and Adrx+Dexa+PTT (adrenalectomized with dexamethasone supplemented with palm tocotrienol). Bloods were taken prior to sacrifice for serum biomarkers and either tibia or femur was harvested for bone biomarkers, gene expressions analysis and histological studies. A double-blinded experiment was performed to calculate the number of total cells (osteoblasts and osteoclast) with intact nuclei within five fields of view. While serum osteocalcin and gene expression did not show any changes, CTX level was increased in the Adrx+Dexa group but reduced after the supplementation of palm tocotrienol. Supplementation of palm tocotrienol also significantly decrease the osteoclast population (p<0.05) compared to Adrx+Dexa group. In contrast, osteoblast population showed no significant difference across the groups. The result showed that palm tocotrienol acts by modulating the osteoclasts but not osteoblast, which revert the dynamics of bone cells population in the osteoporotic bone to its pre-osteoporotic levels. Supplements of tocotrienol in daily diet may be beneficial in preventing osteoporosis. Penerbit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12655/1/16%20Mohamad%20Fairuz%20Yahaya.pdf Mohamad Fairuz Yahaya, and Azizi Zainodin, and Reinukaa Pupathy, and Ong, Edwin Hock Min and Nurul Husna Abu Bakar, and Nor Azimah Zamri, and Halim Ismail, and Elvy Suhana Mohd Ramli, (2018) The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model. Sains Malaysiana, 47 (11). pp. 2731-2739. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A balanced interaction between osteoblast and osteoclast plays a major role in maintaining bone strength and structural integrity. Tocotrienol, a palm derivative with potent antioxidant properties showed an anti-osteoporotic effect but little is known about its mechanism of action. Hence, this research was conducted to determine the effects of palm tocotrienol on the surface osteoblast and osteoclast of the the glucocorticoid-induced osteoporotic bones. The study involved 40 male Sprague-Dawley rats weighing 250-300 g which were randomly divided into an equal number of Baseline, Sham, Adrx+Dexa (adrenalectomized with dexamethasone), Adrx+Dexa+ATF (adrenalectomized with dexamethasone supplemented with α-tocopherol) and Adrx+Dexa+PTT (adrenalectomized with dexamethasone supplemented with palm tocotrienol). Bloods were taken prior to sacrifice for serum biomarkers and either tibia or femur was harvested for bone biomarkers, gene expressions analysis and histological studies. A double-blinded experiment was performed to calculate the number of total cells (osteoblasts and osteoclast) with intact nuclei within five fields of view. While serum osteocalcin and gene expression did not show any changes, CTX level was increased in the Adrx+Dexa group but reduced after the supplementation of palm tocotrienol. Supplementation of palm tocotrienol also significantly decrease the osteoclast population (p<0.05) compared to Adrx+Dexa group. In contrast, osteoblast population showed no significant difference across the groups. The result showed that palm tocotrienol acts by modulating the osteoclasts but not osteoblast, which revert the dynamics of bone cells population in the osteoporotic bone to its pre-osteoporotic levels. Supplements of tocotrienol in daily diet may be beneficial in preventing osteoporosis.
format	Article
author	Mohamad Fairuz Yahaya, Azizi Zainodin, Reinukaa Pupathy, Ong, Edwin Hock Min Nurul Husna Abu Bakar, Nor Azimah Zamri, Halim Ismail, Elvy Suhana Mohd Ramli,
spellingShingle	Mohamad Fairuz Yahaya, Azizi Zainodin, Reinukaa Pupathy, Ong, Edwin Hock Min Nurul Husna Abu Bakar, Nor Azimah Zamri, Halim Ismail, Elvy Suhana Mohd Ramli, The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
author_facet	Mohamad Fairuz Yahaya, Azizi Zainodin, Reinukaa Pupathy, Ong, Edwin Hock Min Nurul Husna Abu Bakar, Nor Azimah Zamri, Halim Ismail, Elvy Suhana Mohd Ramli,
author_sort	Mohamad Fairuz Yahaya,
title	The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
title_short	The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
title_full	The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
title_fullStr	The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
title_full_unstemmed	The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
title_sort	effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12655/ http://journalarticle.ukm.my/12655/ http://journalarticle.ukm.my/12655/1/16%20Mohamad%20Fairuz%20Yahaya.pdf
first_indexed	2023-09-18T20:03:06Z
last_indexed	2023-09-18T20:03:06Z
_version_	1777406979422027776

The effect of palm tocotrienol on surface osteoblast and osteoclast in excess glucocorticoid osteoporotic rat model

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