Induction of polyploid tillered onion using colchicine and pendimethalin

The shoot tip explants of the tillered onion line Z-018 were used as experimental materials. The effects of colchicine and pendimethalin on the induction of polyploid tillered onion were studied in vitro with different concentrations and treatment time. Treatment with 250 μmol/L colchicine for 4 day...

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Main Authors:	Ren, Jian, Wu, Xiaoxu, Song, Ce, Yu Liang, Yu, Gao, Wenzheng, Wang, Yong
Format:	Article
Language:	English
Published:	Penebit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12642/ http://journalarticle.ukm.my/12642/ http://journalarticle.ukm.my/12642/1/04%20Jian%20Ren.pdf

id	ukm-12642
recordtype	eprints
spelling	ukm-126422019-03-17T11:17:27Z http://journalarticle.ukm.my/12642/ Induction of polyploid tillered onion using colchicine and pendimethalin Ren, Jian Wu, Xiaoxu Song, Ce Yu Liang, Yu Gao, Wenzheng Wang, Yong The shoot tip explants of the tillered onion line Z-018 were used as experimental materials. The effects of colchicine and pendimethalin on the induction of polyploid tillered onion were studied in vitro with different concentrations and treatment time. Treatment with 250 μmol/L colchicine for 4 days caused a variant rate of 42.22% and a mortality rate of 36.67%, whereas treatment with 30 μmol/L pendimethalin for 6 days caused a variant rate of 41.11% and a mortality rate of 24.44%. Subsequent cytological identification showed that tetraploid plants were successfully induced by both mutagens. Compared with the diploid plants, the tetraploid plants exhibited slower growth, thicker leaves, stems and roots, larger stomatal apparatus and increased number of chloroplasts. In addition, the obtained tetraploid plants have thicker tubular leaves, decreased number of tillers, larger bulbs and increased yield of the products. This provides the basis for future research on tillered onion germplasm resources. Penebit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12642/1/04%20Jian%20Ren.pdf Ren, Jian and Wu, Xiaoxu and Song, Ce and Yu Liang, Yu and Gao, Wenzheng and Wang, Yong (2018) Induction of polyploid tillered onion using colchicine and pendimethalin. Sains Malaysiana, 47 (11). pp. 2617-2624. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The shoot tip explants of the tillered onion line Z-018 were used as experimental materials. The effects of colchicine and pendimethalin on the induction of polyploid tillered onion were studied in vitro with different concentrations and treatment time. Treatment with 250 μmol/L colchicine for 4 days caused a variant rate of 42.22% and a mortality rate of 36.67%, whereas treatment with 30 μmol/L pendimethalin for 6 days caused a variant rate of 41.11% and a mortality rate of 24.44%. Subsequent cytological identification showed that tetraploid plants were successfully induced by both mutagens. Compared with the diploid plants, the tetraploid plants exhibited slower growth, thicker leaves, stems and roots, larger stomatal apparatus and increased number of chloroplasts. In addition, the obtained tetraploid plants have thicker tubular leaves, decreased number of tillers, larger bulbs and increased yield of the products. This provides the basis for future research on tillered onion germplasm resources.
format	Article
author	Ren, Jian Wu, Xiaoxu Song, Ce Yu Liang, Yu Gao, Wenzheng Wang, Yong
spellingShingle	Ren, Jian Wu, Xiaoxu Song, Ce Yu Liang, Yu Gao, Wenzheng Wang, Yong Induction of polyploid tillered onion using colchicine and pendimethalin
author_facet	Ren, Jian Wu, Xiaoxu Song, Ce Yu Liang, Yu Gao, Wenzheng Wang, Yong
author_sort	Ren, Jian
title	Induction of polyploid tillered onion using colchicine and pendimethalin
title_short	Induction of polyploid tillered onion using colchicine and pendimethalin
title_full	Induction of polyploid tillered onion using colchicine and pendimethalin
title_fullStr	Induction of polyploid tillered onion using colchicine and pendimethalin
title_full_unstemmed	Induction of polyploid tillered onion using colchicine and pendimethalin
title_sort	induction of polyploid tillered onion using colchicine and pendimethalin
publisher	Penebit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12642/ http://journalarticle.ukm.my/12642/ http://journalarticle.ukm.my/12642/1/04%20Jian%20Ren.pdf
first_indexed	2023-09-18T20:03:04Z
last_indexed	2023-09-18T20:03:04Z
_version_	1777406977646788608

Induction of polyploid tillered onion using colchicine and pendimethalin

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