Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application

Currently, fetal bovine serum (FBS) have been widely use in culture media to promote human cell proliferation. However, the usage of FBS for cell therapy in clinical application was associated with the risk of viral and prion transmission as well as immune rejection. To provide an option for this ri...

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Main Authors:	Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong, Tzeng Lin, Liau, Ling Ling, Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chia, Kien Hui
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12506/ http://journalarticle.ukm.my/12506/ http://journalarticle.ukm.my/12506/1/13%20Adila%20A%20Hamid.pdf

id	ukm-12506
recordtype	eprints
spelling	ukm-125062019-01-28T15:21:49Z http://journalarticle.ukm.my/12506/ Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong, Tzeng Lin Liau, Ling Ling Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chia, Kien Hui Currently, fetal bovine serum (FBS) have been widely use in culture media to promote human cell proliferation. However, the usage of FBS for cell therapy in clinical application was associated with the risk of viral and prion transmission as well as immune rejection. To provide an option for this risk, this study was conducted to determine the effect of adipose derived stem cells (ADSCs) co-culture with chondrocyte in promoting cell proliferation and chondrogenesis toward FBS free condition. ADSCs co-cultured with chondrocyte at the ratio of 1:1, 2:1 and 1:2 were tested. Cell morphology changes, cell proliferation and gene expression level of stemness (Oct4, FGF-4, Nanog) and chondrogenic (Collagen Type II, ACP) were assessed. The results showed ADSCs in all co-culture groups changed morphology from fibroblastic spindle to polygonal shape which resembled chondrocytes. The morphological changes were accompanied with increased expression of chondrogenic genes; denoted chondrogenesis process. While maintaining expression of stemness genes indicated continuation of cell proliferation. From the three co-culture groups tested; ADSCs and chondrocytes (1:1 ratio) have been shown to exert better effects in promoting cell proliferation and chondrogenesis. In conclusion, ADSCs could replace FBS to grow sufficient number of chondrogenic cells to repair cartilage injury in the near future. Further in vivo study should be performed to test the effectiveness of this co-culture technique in cartilage injury repair. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12506/1/13%20Adila%20A%20Hamid.pdf Adila A. Hamid, and Satish Vaarman Jeyabalan, and Aleza Omar, and Nik Zattil Hanan Mohd Yasin, and Wong, Tzeng Lin and Liau, Ling Ling and Nur Azurah Abdul Ghani, and Aneeza Khairiyah Wan Hamizan, and Chia, Kien Hui (2018) Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application. Sains Malaysiana, 47 (10). pp. 2369-2379. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Currently, fetal bovine serum (FBS) have been widely use in culture media to promote human cell proliferation. However, the usage of FBS for cell therapy in clinical application was associated with the risk of viral and prion transmission as well as immune rejection. To provide an option for this risk, this study was conducted to determine the effect of adipose derived stem cells (ADSCs) co-culture with chondrocyte in promoting cell proliferation and chondrogenesis toward FBS free condition. ADSCs co-cultured with chondrocyte at the ratio of 1:1, 2:1 and 1:2 were tested. Cell morphology changes, cell proliferation and gene expression level of stemness (Oct4, FGF-4, Nanog) and chondrogenic (Collagen Type II, ACP) were assessed. The results showed ADSCs in all co-culture groups changed morphology from fibroblastic spindle to polygonal shape which resembled chondrocytes. The morphological changes were accompanied with increased expression of chondrogenic genes; denoted chondrogenesis process. While maintaining expression of stemness genes indicated continuation of cell proliferation. From the three co-culture groups tested; ADSCs and chondrocytes (1:1 ratio) have been shown to exert better effects in promoting cell proliferation and chondrogenesis. In conclusion, ADSCs could replace FBS to grow sufficient number of chondrogenic cells to repair cartilage injury in the near future. Further in vivo study should be performed to test the effectiveness of this co-culture technique in cartilage injury repair.
format	Article
author	Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong, Tzeng Lin Liau, Ling Ling Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chia, Kien Hui
spellingShingle	Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong, Tzeng Lin Liau, Ling Ling Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chia, Kien Hui Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
author_facet	Adila A. Hamid, Satish Vaarman Jeyabalan, Aleza Omar, Nik Zattil Hanan Mohd Yasin, Wong, Tzeng Lin Liau, Ling Ling Nur Azurah Abdul Ghani, Aneeza Khairiyah Wan Hamizan, Chia, Kien Hui
author_sort	Adila A. Hamid,
title	Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
title_short	Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
title_full	Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
title_fullStr	Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
title_full_unstemmed	Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
title_sort	chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12506/ http://journalarticle.ukm.my/12506/ http://journalarticle.ukm.my/12506/1/13%20Adila%20A%20Hamid.pdf
first_indexed	2023-09-18T20:02:46Z
last_indexed	2023-09-18T20:02:46Z
_version_	1777406958620377088

Chondrogenesis of adipose-derived stem cells with chondrocytes in low serum towards clinical application

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