Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis

The limitation of self-repair and proliferation capacity of chondrocytes in cartilage reconstruction lead to alternative search of cell source that can improve the auricular regeneration. Human adipose-derived stem cells (HADSC) are an alternative cell source that have unique characteristics to self...

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Main Authors:	Siti Nurhadis Che Omar, Goh, Bee See, Muhammad Azhan Ubaidah, Khairul Anuar Khairoji, Shamsul Sulaiman, Lokman Saim, Chua, Kien-Hui
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12504/ http://journalarticle.ukm.my/12504/ http://journalarticle.ukm.my/12504/1/11%20Siti%20Nurhadis%20Che%20Omar.pdf

id	ukm-12504
recordtype	eprints
spelling	ukm-125042019-01-28T15:20:16Z http://journalarticle.ukm.my/12504/ Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis Siti Nurhadis Che Omar, Goh, Bee See Muhammad Azhan Ubaidah, Khairul Anuar Khairoji, Shamsul Sulaiman, Lokman Saim, Chua, Kien-Hui The limitation of self-repair and proliferation capacity of chondrocytes in cartilage reconstruction lead to alternative search of cell source that can improve the auricular regeneration. Human adipose-derived stem cells (HADSC) are an alternative cell source that have unique characteristics to self-renew and differentiate into various tissues making it suitable for cell therapy and tissue engineering. This study aimed to examine the chondrogenic differentiation potential of (HADSC) in monolayer culture by the presence of different transforming growth factor beta’s, TFG-β1, -β2 and -β3. HADSC at passage 3 (1.5 × 105 cell/mL) were cultured in chondrogenic medium containing 5 ng/mL of different transforming growth factor beta’s, TFG-β1, -β2 and -β3 for 7, 14 and 21 days. Data analysis was evaluated based on the growth rate of cells, cells morphological changed, production of collagen type II and glycosaminoglycan sulphate (sGAG). The quantitative RT-PCR was carried out to determine the chondrogenic, fibrogenic and hypertrophic gene expression levels. Differentiation of HADSC into chondrocytes using TFG-β indicates the occurrence of the chondrogenesis process. The best chondrogenic differentiation was observed in HADSC induced by TFG-β3 through the chondrocytes-like cells morphology with cells aggregation and high production of proteoglycan matrices compared to other TGF-βs groups. Additionally, the expression of chondrocytes-specific genes such as Type II collagen, Aggrecan core protein, Elastin and Sox 9 was high. In conclusion, this study has showed that TGF-β3 is the potential growth factor in producing chondrogenic cells for auricular cartilage tissue engineering. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12504/1/11%20Siti%20Nurhadis%20Che%20Omar.pdf Siti Nurhadis Che Omar, and Goh, Bee See and Muhammad Azhan Ubaidah, and Khairul Anuar Khairoji, and Shamsul Sulaiman, and Lokman Saim, and Chua, Kien-Hui (2018) Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis. Sains Malaysiana, 47 (10). pp. 2349-2358. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The limitation of self-repair and proliferation capacity of chondrocytes in cartilage reconstruction lead to alternative search of cell source that can improve the auricular regeneration. Human adipose-derived stem cells (HADSC) are an alternative cell source that have unique characteristics to self-renew and differentiate into various tissues making it suitable for cell therapy and tissue engineering. This study aimed to examine the chondrogenic differentiation potential of (HADSC) in monolayer culture by the presence of different transforming growth factor beta’s, TFG-β1, -β2 and -β3. HADSC at passage 3 (1.5 × 105 cell/mL) were cultured in chondrogenic medium containing 5 ng/mL of different transforming growth factor beta’s, TFG-β1, -β2 and -β3 for 7, 14 and 21 days. Data analysis was evaluated based on the growth rate of cells, cells morphological changed, production of collagen type II and glycosaminoglycan sulphate (sGAG). The quantitative RT-PCR was carried out to determine the chondrogenic, fibrogenic and hypertrophic gene expression levels. Differentiation of HADSC into chondrocytes using TFG-β indicates the occurrence of the chondrogenesis process. The best chondrogenic differentiation was observed in HADSC induced by TFG-β3 through the chondrocytes-like cells morphology with cells aggregation and high production of proteoglycan matrices compared to other TGF-βs groups. Additionally, the expression of chondrocytes-specific genes such as Type II collagen, Aggrecan core protein, Elastin and Sox 9 was high. In conclusion, this study has showed that TGF-β3 is the potential growth factor in producing chondrogenic cells for auricular cartilage tissue engineering.
format	Article
author	Siti Nurhadis Che Omar, Goh, Bee See Muhammad Azhan Ubaidah, Khairul Anuar Khairoji, Shamsul Sulaiman, Lokman Saim, Chua, Kien-Hui
spellingShingle	Siti Nurhadis Che Omar, Goh, Bee See Muhammad Azhan Ubaidah, Khairul Anuar Khairoji, Shamsul Sulaiman, Lokman Saim, Chua, Kien-Hui Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
author_facet	Siti Nurhadis Che Omar, Goh, Bee See Muhammad Azhan Ubaidah, Khairul Anuar Khairoji, Shamsul Sulaiman, Lokman Saim, Chua, Kien-Hui
author_sort	Siti Nurhadis Che Omar,
title	Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
title_short	Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
title_full	Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
title_fullStr	Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
title_full_unstemmed	Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
title_sort	transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12504/ http://journalarticle.ukm.my/12504/ http://journalarticle.ukm.my/12504/1/11%20Siti%20Nurhadis%20Che%20Omar.pdf
first_indexed	2023-09-18T20:02:45Z
last_indexed	2023-09-18T20:02:45Z
_version_	1777406958329921536

Transforming growth factor beta 3 induced human adipose-derived stem cells for auricular chondrogenesis

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