Effects of PLGA nanofibre on osteoarthritic chondrocytes

Chondrocytes obtained from osteoarthritis (OA) joints has been recognized as an abnormal cell; however, it’s proven to have potential in supporting cartilage regeneration. We have isolated chondrocytes from OA joints (OAC) and expanded chondrocytes growth medium (CGM). The growth kinetic, immunophen...

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Main Authors:	B.S. Shamsul, Shiplu Roy Chowdhury, Ruszymah Haji Idrus, Nor Hamdan Mohamad Yahaya
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12502/ http://journalarticle.ukm.my/12502/ http://journalarticle.ukm.my/12502/1/09%20B.S.%20Shamsul.pdf

id	ukm-12502
recordtype	eprints
spelling	ukm-125022019-01-28T15:18:24Z http://journalarticle.ukm.my/12502/ Effects of PLGA nanofibre on osteoarthritic chondrocytes B.S. Shamsul, Shiplu Roy Chowdhury, Ruszymah Haji Idrus, Nor Hamdan Mohamad Yahaya, Chondrocytes obtained from osteoarthritis (OA) joints has been recognized as an abnormal cell; however, it’s proven to have potential in supporting cartilage regeneration. We have isolated chondrocytes from OA joints (OAC) and expanded chondrocytes growth medium (CGM). The growth kinetic, immunophenotyping and cell multilineage differentiation were analyzed to confirm the OAC stemness. The optimal condition to developed PLGA nanofibre with ratio 50:50 were 20% concentration of PLGA, flow rate with 0.3 mL/h, 10 kv voltage and 10 cm distance from nozzle to the collector. The toxicity level, scanning electron microscopy (SEM) and q-PCR analysis was performed in the present study. OAC fulfills the minimal criteria to be known to have stem cell as the cell easily adheres to the culture plate, shows high expression (≥95%) for CD13, CD29, CD44, CD73 and CD90 and less expression (≤2%) for CD45 and HLA-DR and potentially induced to mesodermal multilineage, which is osteocytes, adipocytes and chondrocytes. Toxicity test showed no adverse effect of PLGA towards the cell. Based on the cell-PLGA nanofibre interaction, difference in fibre size will influence the proliferation of the cell. Nanofibres with 100 nm in size showed high proliferation of OAC and better gene and protein expression compared to monolayer culture. Thus, we concluded that OAC has the potential to be used in cartilage regeneration based on the presence of stem cell markers as similar to the human bone marrow. The cartilage regeneration will be more efficient if OAC cultured on 3D microenvironment as showed in the present study. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12502/1/09%20B.S.%20Shamsul.pdf B.S. Shamsul, and Shiplu Roy Chowdhury, and Ruszymah Haji Idrus, and Nor Hamdan Mohamad Yahaya, (2018) Effects of PLGA nanofibre on osteoarthritic chondrocytes. Sains Malaysiana, 47 (10). pp. 2325-2336. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Chondrocytes obtained from osteoarthritis (OA) joints has been recognized as an abnormal cell; however, it’s proven to have potential in supporting cartilage regeneration. We have isolated chondrocytes from OA joints (OAC) and expanded chondrocytes growth medium (CGM). The growth kinetic, immunophenotyping and cell multilineage differentiation were analyzed to confirm the OAC stemness. The optimal condition to developed PLGA nanofibre with ratio 50:50 were 20% concentration of PLGA, flow rate with 0.3 mL/h, 10 kv voltage and 10 cm distance from nozzle to the collector. The toxicity level, scanning electron microscopy (SEM) and q-PCR analysis was performed in the present study. OAC fulfills the minimal criteria to be known to have stem cell as the cell easily adheres to the culture plate, shows high expression (≥95%) for CD13, CD29, CD44, CD73 and CD90 and less expression (≤2%) for CD45 and HLA-DR and potentially induced to mesodermal multilineage, which is osteocytes, adipocytes and chondrocytes. Toxicity test showed no adverse effect of PLGA towards the cell. Based on the cell-PLGA nanofibre interaction, difference in fibre size will influence the proliferation of the cell. Nanofibres with 100 nm in size showed high proliferation of OAC and better gene and protein expression compared to monolayer culture. Thus, we concluded that OAC has the potential to be used in cartilage regeneration based on the presence of stem cell markers as similar to the human bone marrow. The cartilage regeneration will be more efficient if OAC cultured on 3D microenvironment as showed in the present study.
format	Article
author	B.S. Shamsul, Shiplu Roy Chowdhury, Ruszymah Haji Idrus, Nor Hamdan Mohamad Yahaya,
spellingShingle	B.S. Shamsul, Shiplu Roy Chowdhury, Ruszymah Haji Idrus, Nor Hamdan Mohamad Yahaya, Effects of PLGA nanofibre on osteoarthritic chondrocytes
author_facet	B.S. Shamsul, Shiplu Roy Chowdhury, Ruszymah Haji Idrus, Nor Hamdan Mohamad Yahaya,
author_sort	B.S. Shamsul,
title	Effects of PLGA nanofibre on osteoarthritic chondrocytes
title_short	Effects of PLGA nanofibre on osteoarthritic chondrocytes
title_full	Effects of PLGA nanofibre on osteoarthritic chondrocytes
title_fullStr	Effects of PLGA nanofibre on osteoarthritic chondrocytes
title_full_unstemmed	Effects of PLGA nanofibre on osteoarthritic chondrocytes
title_sort	effects of plga nanofibre on osteoarthritic chondrocytes
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12502/ http://journalarticle.ukm.my/12502/ http://journalarticle.ukm.my/12502/1/09%20B.S.%20Shamsul.pdf
first_indexed	2023-09-18T20:02:45Z
last_indexed	2023-09-18T20:02:45Z
_version_	1777406958037368832

Effects of PLGA nanofibre on osteoarthritic chondrocytes

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