The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan

PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. How...

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Main Authors:	Nadiya Akmal Baharum, Rofina Yasmin Othman, Yusmin Mohd-Yusuf, Tan, Boon Chin, Kamilatulhusna Zaidi, Norzulaani Khalid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12498/ http://journalarticle.ukm.my/12498/ http://journalarticle.ukm.my/12498/1/05%20Nadiya%20Akmal%20Baharum.pdf

id	ukm-12498
recordtype	eprints
spelling	ukm-124982019-01-28T08:59:37Z http://journalarticle.ukm.my/12498/ The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan Nadiya Akmal Baharum, Rofina Yasmin Othman, Yusmin Mohd-Yusuf, Tan, Boon Chin Kamilatulhusna Zaidi, Norzulaani Khalid, PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12498/1/05%20Nadiya%20Akmal%20Baharum.pdf Nadiya Akmal Baharum, and Rofina Yasmin Othman, and Yusmin Mohd-Yusuf, and Tan, Boon Chin and Kamilatulhusna Zaidi, and Norzulaani Khalid, (2018) The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan. Sains Malaysiana, 47 (10). pp. 2291-2300. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num10_2018/contentsVol47num10_2018.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants.
format	Article
author	Nadiya Akmal Baharum, Rofina Yasmin Othman, Yusmin Mohd-Yusuf, Tan, Boon Chin Kamilatulhusna Zaidi, Norzulaani Khalid,
spellingShingle	Nadiya Akmal Baharum, Rofina Yasmin Othman, Yusmin Mohd-Yusuf, Tan, Boon Chin Kamilatulhusna Zaidi, Norzulaani Khalid, The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
author_facet	Nadiya Akmal Baharum, Rofina Yasmin Othman, Yusmin Mohd-Yusuf, Tan, Boon Chin Kamilatulhusna Zaidi, Norzulaani Khalid,
author_sort	Nadiya Akmal Baharum,
title	The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
title_short	The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
title_full	The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
title_fullStr	The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
title_full_unstemmed	The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
title_sort	effect of pathogenesis-related 10 (pr-10) gene on the progression of fusarium wilt in musa acuminata cv. berangan
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12498/ http://journalarticle.ukm.my/12498/ http://journalarticle.ukm.my/12498/1/05%20Nadiya%20Akmal%20Baharum.pdf
first_indexed	2023-09-18T20:02:45Z
last_indexed	2023-09-18T20:02:45Z
_version_	1777406957450166272

The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan

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