Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells

Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the p...

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Main Authors:	Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng, Norazrina Azmi
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12404/ http://journalarticle.ukm.my/12404/ http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf

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spelling	ukm-124042018-12-07T23:38:37Z http://journalarticle.ukm.my/12404/ Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng Norazrina Azmi, Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the present study, the neuroprotective effects of Ocimum basilicum var. thyrsiflora against hydrogen peroxide (H2O2)-induced oxidative stress in SK-N-SH neuroblastoma cells were evaluated. The exposure of SK-N-SH cells to 50 μM H2O2 for 24 h induced cytotoxicity and apoptosis as measured by cell viability and flow cytometry, respectively. Pretreatment with ethyl acetate (ObEA) fraction at 3.1-25 μg/mL showed the highest protection against H2O2-induced cell death compared to other fractions and crude extract by increasing cell viability and reducing apoptosis. The evaluation of antioxidant capacity via 1, 1-diphenyl-2-picrylahydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays showed ObEA possessed the highest antioxidative properties. The intracellular reactive oxygen species (ROS) production of H2O2 in untreated cells increased by 2.39-fold compared to the control and was significantly attenuated by the 2 h pre-treatment of O. basilicum (p<0.05). The reduction in intracellular superoxide dismutase (SOD) induced by H2O2 was also abrogated by the pretreatment of O. basilicum. These findings suggested that O. basilicum is potentially neuroprotective against oxidative damage in neuronal cells by scavenging free radicals, restoring SOD activities and eventually prevent cell death. Penerbit Universiti Kebangsaan Malaysia 2018-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf Manali Haniti Mohd-Zahid, and Juriyati Jalil, and Chan, Kok Meng and Norazrina Azmi, (2018) Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells. Sains Malaysiana, 47 (9). pp. 2129-2139. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the present study, the neuroprotective effects of Ocimum basilicum var. thyrsiflora against hydrogen peroxide (H2O2)-induced oxidative stress in SK-N-SH neuroblastoma cells were evaluated. The exposure of SK-N-SH cells to 50 μM H2O2 for 24 h induced cytotoxicity and apoptosis as measured by cell viability and flow cytometry, respectively. Pretreatment with ethyl acetate (ObEA) fraction at 3.1-25 μg/mL showed the highest protection against H2O2-induced cell death compared to other fractions and crude extract by increasing cell viability and reducing apoptosis. The evaluation of antioxidant capacity via 1, 1-diphenyl-2-picrylahydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays showed ObEA possessed the highest antioxidative properties. The intracellular reactive oxygen species (ROS) production of H2O2 in untreated cells increased by 2.39-fold compared to the control and was significantly attenuated by the 2 h pre-treatment of O. basilicum (p<0.05). The reduction in intracellular superoxide dismutase (SOD) induced by H2O2 was also abrogated by the pretreatment of O. basilicum. These findings suggested that O. basilicum is potentially neuroprotective against oxidative damage in neuronal cells by scavenging free radicals, restoring SOD activities and eventually prevent cell death.
format	Article
author	Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng Norazrina Azmi,
spellingShingle	Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng Norazrina Azmi, Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
author_facet	Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng Norazrina Azmi,
author_sort	Manali Haniti Mohd-Zahid,
title	Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_short	Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_full	Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_fullStr	Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_full_unstemmed	Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_sort	neuroprotective effects of ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in sk-n-sh neuroblastoma cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12404/ http://journalarticle.ukm.my/12404/ http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf
first_indexed	2023-09-18T20:02:32Z
last_indexed	2023-09-18T20:02:32Z
_version_	1777406944047267840

Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells

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