Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature

The Meliponini honey is one type of stingless bee honey. Although honey has been widely used as a natural remedy, understanding on how storage affect the microbiological quality and pH in stingless honey is still scarce. This study was carried out to determine the microbial quality of honey produc...

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Main Authors: Mohd Nizam Lani, Amirah Hazirah Zainudin, Shamsul Bahri Abdul Razak, Azlina Mansor, Zaiton Hassan
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/12364/
http://journalarticle.ukm.my/12364/
http://journalarticle.ukm.my/12364/1/46_03_12.pdf
id ukm-12364
recordtype eprints
spelling ukm-123642018-11-30T11:03:29Z http://journalarticle.ukm.my/12364/ Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature Mohd Nizam Lani, Amirah Hazirah Zainudin, Shamsul Bahri Abdul Razak, Azlina Mansor, Zaiton Hassan, The Meliponini honey is one type of stingless bee honey. Although honey has been widely used as a natural remedy, understanding on how storage affect the microbiological quality and pH in stingless honey is still scarce. This study was carried out to determine the microbial quality of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica during ambient storage for 6 weeks. The changes in microbiological analysis was determined for Aerobic Plate Count (APC), yeast count and Bacillus count. Lactic acid bacteria (LAB) were isolated using three types of media, namely, MRS agar, MRS agar with 0.8% CaCO3 and MRS agar with 1% glucose. Detection of Clostridium was done using anaerobic egg-yolk agar and cook meat medium. Results showed that Aerobic Plate Count ranged between 102 to 105 CFU/g for both samples. However, yeast count showed the range between 102 to 106 CFU/g, which higher than APC. Both honey samples showed low in Bacillus count. The LAB counts on three media were significantly higher for the first month of storage, then decreased gradually after fifth week. Storage of honey had reduced the pH in both honey samples to pH 2. Clostridium was not detected in all samples. Penerbit Universiti Kebangsaan Malaysia 2017-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12364/1/46_03_12.pdf Mohd Nizam Lani, and Amirah Hazirah Zainudin, and Shamsul Bahri Abdul Razak, and Azlina Mansor, and Zaiton Hassan, (2017) Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature. Malaysian Applied Biology, 46 (3). pp. 89-96. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=674&catid=59:current-view&Itemid=56
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institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description The Meliponini honey is one type of stingless bee honey. Although honey has been widely used as a natural remedy, understanding on how storage affect the microbiological quality and pH in stingless honey is still scarce. This study was carried out to determine the microbial quality of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica during ambient storage for 6 weeks. The changes in microbiological analysis was determined for Aerobic Plate Count (APC), yeast count and Bacillus count. Lactic acid bacteria (LAB) were isolated using three types of media, namely, MRS agar, MRS agar with 0.8% CaCO3 and MRS agar with 1% glucose. Detection of Clostridium was done using anaerobic egg-yolk agar and cook meat medium. Results showed that Aerobic Plate Count ranged between 102 to 105 CFU/g for both samples. However, yeast count showed the range between 102 to 106 CFU/g, which higher than APC. Both honey samples showed low in Bacillus count. The LAB counts on three media were significantly higher for the first month of storage, then decreased gradually after fifth week. Storage of honey had reduced the pH in both honey samples to pH 2. Clostridium was not detected in all samples.
format Article
author Mohd Nizam Lani,
Amirah Hazirah Zainudin,
Shamsul Bahri Abdul Razak,
Azlina Mansor,
Zaiton Hassan,
spellingShingle Mohd Nizam Lani,
Amirah Hazirah Zainudin,
Shamsul Bahri Abdul Razak,
Azlina Mansor,
Zaiton Hassan,
Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
author_facet Mohd Nizam Lani,
Amirah Hazirah Zainudin,
Shamsul Bahri Abdul Razak,
Azlina Mansor,
Zaiton Hassan,
author_sort Mohd Nizam Lani,
title Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
title_short Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
title_full Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
title_fullStr Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
title_full_unstemmed Microbiological quality and pH changes of honey produced by stingless bees, Heterotrigona itama and Geniotrigona thoracica stored at ambient temperature
title_sort microbiological quality and ph changes of honey produced by stingless bees, heterotrigona itama and geniotrigona thoracica stored at ambient temperature
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/12364/
http://journalarticle.ukm.my/12364/
http://journalarticle.ukm.my/12364/1/46_03_12.pdf
first_indexed 2023-09-18T20:02:26Z
last_indexed 2023-09-18T20:02:26Z
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