Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance

Non-typhoidal Salmonella is considered as the leading cause of foodborne illness and it has been associated with highprofile outbreaks in many groups of foods. This work examines the contribution of phenotypic properties related to survival (biofilm formation) and how these are linked with the gen...

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Main Authors:	Wan Zawiah Wan Abdullah, Mackey, Bernard M., Karatzas, Kimon-Andreas G.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12360/ http://journalarticle.ukm.my/12360/ http://journalarticle.ukm.my/12360/1/46_03_08.pdf

id	ukm-12360
recordtype	eprints
spelling	ukm-123602018-11-30T10:56:51Z http://journalarticle.ukm.my/12360/ Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance Wan Zawiah Wan Abdullah, Mackey, Bernard M. Karatzas, Kimon-Andreas G. Non-typhoidal Salmonella is considered as the leading cause of foodborne illness and it has been associated with highprofile outbreaks in many groups of foods. This work examines the contribution of phenotypic properties related to survival (biofilm formation) and how these are linked with the genetic and functional variability of rpoS gene and RpoS status respectively in Salmonella enterica. The test strains were Salmonella serovars Anatum, Enteritidis (466), Enteritidis (496), Hadar, Heidelberg, Montevideo, Newport and Virchow and two Typhimurium strains previously characterised as either RpoSpositive or RpoS-negative. RpoS status was found to affect biofilm formation. The capability of Salmonella to resist stress and survive under unfavourable conditions can vary between strains. We confirmed that the two strains that were previously sensitive to various stresses harboured significant mutations in the rpoS gene. The rpoS sequencing not only confirmed a link between RpoS and biofilm formation, but it also revealed a link with differences in the utilisation of carbon sources. The RpoS-negative phenotype was linked with an increased growth under different carbon sources suggesting that a functional RpoS is a burden for growth which is in agreement with the SPANC hypothesis. Penerbit Universiti Kebangsaan Malaysia 2017-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12360/1/46_03_08.pdf Wan Zawiah Wan Abdullah, and Mackey, Bernard M. and Karatzas, Kimon-Andreas G. (2017) Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance. Malaysian Applied Biology, 46 (3). pp. 59-65. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=674&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Non-typhoidal Salmonella is considered as the leading cause of foodborne illness and it has been associated with highprofile outbreaks in many groups of foods. This work examines the contribution of phenotypic properties related to survival (biofilm formation) and how these are linked with the genetic and functional variability of rpoS gene and RpoS status respectively in Salmonella enterica. The test strains were Salmonella serovars Anatum, Enteritidis (466), Enteritidis (496), Hadar, Heidelberg, Montevideo, Newport and Virchow and two Typhimurium strains previously characterised as either RpoSpositive or RpoS-negative. RpoS status was found to affect biofilm formation. The capability of Salmonella to resist stress and survive under unfavourable conditions can vary between strains. We confirmed that the two strains that were previously sensitive to various stresses harboured significant mutations in the rpoS gene. The rpoS sequencing not only confirmed a link between RpoS and biofilm formation, but it also revealed a link with differences in the utilisation of carbon sources. The RpoS-negative phenotype was linked with an increased growth under different carbon sources suggesting that a functional RpoS is a burden for growth which is in agreement with the SPANC hypothesis.
format	Article
author	Wan Zawiah Wan Abdullah, Mackey, Bernard M. Karatzas, Kimon-Andreas G.
spellingShingle	Wan Zawiah Wan Abdullah, Mackey, Bernard M. Karatzas, Kimon-Andreas G. Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
author_facet	Wan Zawiah Wan Abdullah, Mackey, Bernard M. Karatzas, Kimon-Andreas G.
author_sort	Wan Zawiah Wan Abdullah,
title	Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
title_short	Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
title_full	Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
title_fullStr	Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
title_full_unstemmed	Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance
title_sort	biofilm formation of salmonella enterica and the central role of rpos sigma factor in stress resistance
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12360/ http://journalarticle.ukm.my/12360/ http://journalarticle.ukm.my/12360/1/46_03_08.pdf
first_indexed	2023-09-18T20:02:26Z
last_indexed	2023-09-18T20:02:26Z
_version_	1777406937677168640

Biofilm formation of Salmonella enterica and the central role of rpos sigma factor in stress resistance

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