Synthesis of a nanoparticle system for the enhanced accumulation of fluorescently-labelled amino acids encapsulated in monodispersed chitosan nanoparticle system
Poor in vivo bioavailability of nutrient is a major challenge in efficient delivery of nutraceutics. The increased bioavailability of nutraceuticals is prerequisite for efficient absorption by the gastrointestinal system. Nanotechnology-based approaches for nutraceutical applications could potenti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12318/ http://journalarticle.ukm.my/12318/ http://journalarticle.ukm.my/12318/1/46_01_25.pdf |
| Summary: | Poor in vivo bioavailability of nutrient is a major challenge in efficient delivery of nutraceutics. The increased bioavailability
of nutraceuticals is prerequisite for efficient absorption by the gastrointestinal system. Nanotechnology-based approaches for
nutraceutical applications could potentially increase absorption of nutrients and enhance its cellular accumulation due to its
nanosize and promote better in vivo energy biodistribution. However, the dynamics of intracellular cell trafficking of nanoparticles
and nutraceutical release has remain scarcely studied. This study describes a non-efficacious nanoparticle-mediated system for
the encapsulation and delivery of fluorescently-labelled amino acids using tripolyphosphate as a crosslinker. Light scattering
data showed successful formation of particle size as small as 65.69 nm with low polydispersity index (PDI) value of 0.178
at specific volume ratios of chitosan to tripolyphosphate. Following encapsulation, nanoparticle size and PDI value increased
to 182.73 nm and 0.257 respectively discern successful accommodation of the fluorescently-labelled amino acid within its
core. In vitro visualization of amino acids release and accumulation via fluorescence microscopy suggested that encapsulated
amino acids were efficiently accumulated into Vero3 cell cytoplasm at 24 hours post treatment with localization in proximity
to the cell nucleus. These results therefore suggested that the chitosan nanoparticle system developed was able to enhance the
intracellular accumulation of glutamic acids and may serve as a suitable carrier for nutraceuticals delivery. |
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