Encapsulation of miRNA in chitosan nanoparticles as a candidate for an anti-metastatic agent in cancer therapy
MicroRNAs (miRNA) have been utilised as a repressor molecule for metastasis of tumours, as it inhibits fundamental processes related to cellular and physiological pathway of the tumour at the mRNA level. However, therapeutic application of miRNAs is impaired by premature degradation in the extrace...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
|
| Online Access: | http://journalarticle.ukm.my/12317/ http://journalarticle.ukm.my/12317/ http://journalarticle.ukm.my/12317/1/46_01_24.pdf |
| Summary: | MicroRNAs (miRNA) have been utilised as a repressor molecule for metastasis of tumours, as it inhibits fundamental processes
related to cellular and physiological pathway of the tumour at the mRNA level. However, therapeutic application of miRNAs
is impaired by premature degradation in the extracellular environment by endonucleases. This research describes the
optimisation, chemical, and morphological characterisation of nanoparticles for effective encapsulation of miRNA-186 and
evaluate its efficiency as anti-metastatic agent in non-small cell lung carcinoma monolayer. Through ionic gelation methods,
the miRNA was encapsulated in chitosan nanoparticles (CNPs), a drug carrier with high particle stability, low cellular toxicity,
and robust preparation methods. Physiochemical and morphological characterization analysis through light scattering analysis
showed miRNA-CNP sizes below 200 nm, with a low polydispersity index and exhibition of irregular spherical shape of the
nanoparticles synthesised through FESEM analysis. Additionally, in vitro nanoparticle efficacy evaluated through scratch
assay suggests a decrease in invasion ability of cancer cells exhibited by miRNA-CNP. |
|---|