Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii

The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-fol...

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Main Authors:	Mok, Pei Shze, Sudesh Kumar, Liew, Pauline Woan Ying, Jong, Bor Chyan, Najimudin Nazalan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/1/46_01_14.pdf

id	ukm-12307
recordtype	eprints
spelling	ukm-123072018-11-16T11:56:27Z http://journalarticle.ukm.my/12307/ Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii Mok, Pei Shze Sudesh Kumar, Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of 3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium by increasing the sucrose concentration from 20 g/L to 25 g/L. Penerbit Universiti Kebangsaan Malaysia 2017-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12307/1/46_01_14.pdf Mok, Pei Shze and Sudesh Kumar, and Liew, Pauline Woan Ying and Jong, Bor Chyan and Najimudin Nazalan, (2017) Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii. Malaysian Applied Biology, 46 (1). pp. 93-100. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of 3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium by increasing the sucrose concentration from 20 g/L to 25 g/L.
format	Article
author	Mok, Pei Shze Sudesh Kumar, Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan,
spellingShingle	Mok, Pei Shze Sudesh Kumar, Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
author_facet	Mok, Pei Shze Sudesh Kumar, Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan,
author_sort	Mok, Pei Shze
title	Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_short	Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_full	Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_fullStr	Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_full_unstemmed	Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_sort	characterisation of polyhydroxyalkanoate production by mutant azotobacter vinelandii
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/1/46_01_14.pdf
first_indexed	2023-09-18T20:02:18Z
last_indexed	2023-09-18T20:02:18Z
_version_	1777406930006835200

Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii

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