Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-fol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/ http://journalarticle.ukm.my/12307/1/46_01_14.pdf |
| Summary: | The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter
medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of
Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L
compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this
bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its
biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of
3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium
by increasing the sucrose concentration from 20 g/L to 25 g/L. |
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