Dynamic changes of bacterial community during the composting of oil palm empty fruit bunches (OPEFB) as analyzed by denaturing gradient gel electrophoresis (DGGE) analysis
In this study cultivation-independent approach was performed to characterize and understand the bacterial community dynamics during the oil palm empty fruit bunches (OPEFB) composting process. OPEFB was composted in a compost heap of 1m × 1m × 1m. During the process, bacterial community structure...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12306/ http://journalarticle.ukm.my/12306/ http://journalarticle.ukm.my/12306/1/46_01_13.pdf |
| Summary: | In this study cultivation-independent approach was performed to characterize and understand the bacterial community dynamics
during the oil palm empty fruit bunches (OPEFB) composting process. OPEFB was composted in a compost heap of 1m ×
1m × 1m. During the process, bacterial community structure in specific composting time were characterized by denaturing
gradient gel electrophoresis (DGGE) analysis based on the hypervariable V3 region of 16S rRNA gene. The DGGE banding
patterns revealed significant change in the bacterial community structure throughout the composting process. A total of 38
bands were selected for sequencing analysis. Majority of the DGGE bands’ sequences were related to known counterparts
with low percentage of identity indicating novel composting bacterial populations. These DNA sequences were related to
culturable relatives of Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Fibrobacteres and Chloroflexi. In general,
distinctive predomination by Firmicutes followed by succession by Proteobacteria were revealed during the OPEFB composting
process. Information attained in this study may be useful for improving the efficiency of OPEFB composting by indigenous
bacterial population. The diverse sequences from known bacteria indicated novelty in the composting bacterial populations
and potentially their functions. |
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