Variants production of Lentinula edodes spores, monokaryon mycelium and dikaryon mycelium by -irradiation
Lentinula edodes or commonly known as shiitake is a cultivated edible mushroom. This mushroom has been used in pharmaceutical, nutraceutical and cosmeceutical industries. However after years of selective inbreeding, the mushroom suffers from a small genetic pool. In this study, we attempt to produ...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12305/ http://journalarticle.ukm.my/12305/ http://journalarticle.ukm.my/12305/1/46_01_12.pdf |
| Summary: | Lentinula edodes or commonly known as shiitake is a cultivated edible mushroom. This mushroom has been used in
pharmaceutical, nutraceutical and cosmeceutical industries. However after years of selective inbreeding, the mushroom suffers
from a small genetic pool. In this study, we attempt to produce genetic variants of shiitake via γ-irradiation. Shiitake’s spores
and mycelium (both monokaryon (MM) and dikaryon (DM)) were subjected to γ-irradiation (137Cs) at series of doses ranging
from 0 to 4000 Gy. Dose responses were evaluated based on mycelium growth performance and survivability. The γ-irradiation
LD50 for shiitake’s spores, MM, 3rd DM subculture and 9th DM subculture were 795 Gy, 460 Gy, 735 Gy and 1330 Gy,
respectively. Interestingly our result shows those higher subcultures DM are more resistant to γ-irradiation. We observed that
MMs which were exposed to higher dose of γ-irradiation show lower numbers of clamp connections compared to control
indicating that there are genetic compatibility changes between individual hyphal cells within the irradiated shiitake mycelia.
This study revealed the potential of γ-irradiation as a tool to create diversity in shiitake genetic pool towards healthy and
sustainable mushroom crops in the industries. |
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