Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates

Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic...

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Main Authors:	Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12222/ http://journalarticle.ukm.my/12222/ http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf

id	ukm-12222
recordtype	eprints
spelling	ukm-122222018-10-19T21:09:00Z http://journalarticle.ukm.my/12222/ Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop, Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 μg/mg, respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 μg/mg, respectively), and high ergosterol (Candida tropicalis at 22.84 μg/mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf Ahmad Sh. A. Lafi, and Jacinta Santhanam, and Tzar Mohd Nizam Khaithir, and Nur Fashya Musa, and Fahrul Huyop, (2018) Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates. Jurnal Sains Kesihatan Malaysia, 16 (2). pp. 15-21. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/685
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 μg/mg, respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 μg/mg, respectively), and high ergosterol (Candida tropicalis at 22.84 μg/mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples.
format	Article
author	Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop,
spellingShingle	Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop, Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
author_facet	Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop,
author_sort	Ahmad Sh. A. Lafi,
title	Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_short	Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_full	Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_fullStr	Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_full_unstemmed	Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_sort	determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12222/ http://journalarticle.ukm.my/12222/ http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf
first_indexed	2023-09-18T20:02:08Z
last_indexed	2023-09-18T20:02:08Z
_version_	1777406918842646528

Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates

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