Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus

In this work, a preliminary study was conducted to study the effects of different types and concentrations of cosolvents based on the total yield and antioxidants capacity prior to supercritical fluid extraction (SFE) of Orthosiphon stamineus (locally referred as misai kucing). Initially, a comparis...

Full description

Bibliographic Details
Main Authors: Masniza Mohamed@Mahmood, Wan Ramli Wan Daud, Masturah Markom, Che Nurul Ain Nadirah Che Mansor
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12196/
http://journalarticle.ukm.my/12196/
http://journalarticle.ukm.my/12196/1/13%20Masniza%20Mohamed.pdf
id ukm-12196
recordtype eprints
spelling ukm-121962018-10-16T13:32:05Z http://journalarticle.ukm.my/12196/ Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus Masniza Mohamed@Mahmood, Wan Ramli Wan Daud, Masturah Markom, Che Nurul Ain Nadirah Che Mansor, In this work, a preliminary study was conducted to study the effects of different types and concentrations of cosolvents based on the total yield and antioxidants capacity prior to supercritical fluid extraction (SFE) of Orthosiphon stamineus (locally referred as misai kucing). Initially, a comparison was made by cold maceration technique with nine types of different cosolvents, namely water, pure ethanol, 25% (v/v) of ethanol in water, 50% (v/v) of ethanol in water, 75% (v/v) of ethanol in water, pure methanol, 25% (v/v) of methanol in water, 50% (v/v) of methanol in water and 75% (v/v) of methanol in water. The antioxidant capacity was analysed by free radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC) and total flavonoid content (TFC). Aqueous ethanolic solvent of 50% (v/v) ethanol in water showed the highest total yield of extract of 4.64 ± 0.02%. All antioxidant assays of TPC and TFC showed the highest value of 3.42 ± 0.08 mg GAE g−1 extract, 4.7 ± 0.14 mg CAE g−1 extract, respectively and IC50 value for DPPH was 0.625 μg/mL for 50% (v/v) ethanol in water extract. Based on the overall result, ethanolic solvents gave a better result for all antioxidant assays compared to those of methanolic solvents. Using the selected cosolvent, the identification of target compounds, which were rosmarinic acid, eupatorin and sinensetin from supercritical fluid extraction was determined by using HPLC. In conclusion, ethanol-water solvent was efficient in extracting bioactive compounds in O. stamineus and also improved the total yield, thus the usage of ethanolic solvent in different concentrations should be considered for further optimisation of SFE with cosolvent studies. Penerbit Universiti Kebangsaan Malaysia 2018-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12196/1/13%20Masniza%20Mohamed.pdf Masniza Mohamed@Mahmood, and Wan Ramli Wan Daud, and Masturah Markom, and Che Nurul Ain Nadirah Che Mansor, (2018) Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus. Sains Malaysiana, 47 (8). pp. 1741-1747. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description In this work, a preliminary study was conducted to study the effects of different types and concentrations of cosolvents based on the total yield and antioxidants capacity prior to supercritical fluid extraction (SFE) of Orthosiphon stamineus (locally referred as misai kucing). Initially, a comparison was made by cold maceration technique with nine types of different cosolvents, namely water, pure ethanol, 25% (v/v) of ethanol in water, 50% (v/v) of ethanol in water, 75% (v/v) of ethanol in water, pure methanol, 25% (v/v) of methanol in water, 50% (v/v) of methanol in water and 75% (v/v) of methanol in water. The antioxidant capacity was analysed by free radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC) and total flavonoid content (TFC). Aqueous ethanolic solvent of 50% (v/v) ethanol in water showed the highest total yield of extract of 4.64 ± 0.02%. All antioxidant assays of TPC and TFC showed the highest value of 3.42 ± 0.08 mg GAE g−1 extract, 4.7 ± 0.14 mg CAE g−1 extract, respectively and IC50 value for DPPH was 0.625 μg/mL for 50% (v/v) ethanol in water extract. Based on the overall result, ethanolic solvents gave a better result for all antioxidant assays compared to those of methanolic solvents. Using the selected cosolvent, the identification of target compounds, which were rosmarinic acid, eupatorin and sinensetin from supercritical fluid extraction was determined by using HPLC. In conclusion, ethanol-water solvent was efficient in extracting bioactive compounds in O. stamineus and also improved the total yield, thus the usage of ethanolic solvent in different concentrations should be considered for further optimisation of SFE with cosolvent studies.
format Article
author Masniza Mohamed@Mahmood,
Wan Ramli Wan Daud,
Masturah Markom,
Che Nurul Ain Nadirah Che Mansor,
spellingShingle Masniza Mohamed@Mahmood,
Wan Ramli Wan Daud,
Masturah Markom,
Che Nurul Ain Nadirah Che Mansor,
Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
author_facet Masniza Mohamed@Mahmood,
Wan Ramli Wan Daud,
Masturah Markom,
Che Nurul Ain Nadirah Che Mansor,
author_sort Masniza Mohamed@Mahmood,
title Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
title_short Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
title_full Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
title_fullStr Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
title_full_unstemmed Cosolvent selection for supercritical fluid extraction (SFE) of bioactive compounds from Orthosiphon stamineus
title_sort cosolvent selection for supercritical fluid extraction (sfe) of bioactive compounds from orthosiphon stamineus
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12196/
http://journalarticle.ukm.my/12196/
http://journalarticle.ukm.my/12196/1/13%20Masniza%20Mohamed.pdf
first_indexed 2023-09-18T20:02:04Z
last_indexed 2023-09-18T20:02:04Z
_version_ 1777406915155853312