Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression

Oil palm is the major crop grown and cultivated in various Asian countries such as Malaysia, Indonesia and Thailand. The core of oil palm trunk (COPT) consists of high sugar content, hence suitable for synthesis of fine chemicals and biofuels. Increase of sugar content was reported previously during...

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Main Authors:	Marhaini Mostapha, Noorhasmiera Abu Jahar, Kamalrul Azlan Azizan, Sarani Zakaria, Wan Mohd Aizat, Sharifah Nabihah Syed Jaafar
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12131/ http://journalarticle.ukm.my/12131/ http://journalarticle.ukm.my/12131/1/22%20Marhaini%20Mostapha.pdf

id	ukm-12131
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spelling	ukm-121312018-09-24T08:08:15Z http://journalarticle.ukm.my/12131/ Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression Marhaini Mostapha, Noorhasmiera Abu Jahar, Kamalrul Azlan Azizan, Sarani Zakaria, Wan Mohd Aizat, Sharifah Nabihah Syed Jaafar, Oil palm is the major crop grown and cultivated in various Asian countries such as Malaysia, Indonesia and Thailand. The core of oil palm trunk (COPT) consists of high sugar content, hence suitable for synthesis of fine chemicals and biofuels. Increase of sugar content was reported previously during prolonged COPT storage. However, until now, there has been no report on protein profiles during storage. Therefore, in this study, protein expression of the COPT during the storage period of one to six weeks was investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) coupled with optical density quantification and multivariate analyses for measuring differentially expressed proteins. Accordingly, protein bands were subjected to tryptic digestion followed by tandem mass spectrometry (nanoLC-MS/MS) protein identification. The results from SDS-PAGE showed consistent protein bands appearing across the biological replicates ranging from 10.455 to 202.92 kDa molecular weight (MW) regions. The findings from the principal component analysis (PCA) plot illustrated the separation pattern of the proteins at weeks 4 and 5 of storage, which was influenced mainly by the molecular weights of 14.283, 25.543, 29.757, 30.549, 31.511, 34.585 and 84.395 kDa, respectively. The majority of these proteins are identified as those involved in stress- and defense-related, disease resistance, as well as gene/protein expression processes. Indeed, these proteins were mostly upregulated during the later storage period suggesting that long-term storage may influence the molecular regulation of COPT sap. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12131/1/22%20Marhaini%20Mostapha.pdf Marhaini Mostapha, and Noorhasmiera Abu Jahar, and Kamalrul Azlan Azizan, and Sarani Zakaria, and Wan Mohd Aizat, and Sharifah Nabihah Syed Jaafar, (2018) Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression. Sains Malaysiana, 47 (6). pp. 1259-1268. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Oil palm is the major crop grown and cultivated in various Asian countries such as Malaysia, Indonesia and Thailand. The core of oil palm trunk (COPT) consists of high sugar content, hence suitable for synthesis of fine chemicals and biofuels. Increase of sugar content was reported previously during prolonged COPT storage. However, until now, there has been no report on protein profiles during storage. Therefore, in this study, protein expression of the COPT during the storage period of one to six weeks was investigated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) coupled with optical density quantification and multivariate analyses for measuring differentially expressed proteins. Accordingly, protein bands were subjected to tryptic digestion followed by tandem mass spectrometry (nanoLC-MS/MS) protein identification. The results from SDS-PAGE showed consistent protein bands appearing across the biological replicates ranging from 10.455 to 202.92 kDa molecular weight (MW) regions. The findings from the principal component analysis (PCA) plot illustrated the separation pattern of the proteins at weeks 4 and 5 of storage, which was influenced mainly by the molecular weights of 14.283, 25.543, 29.757, 30.549, 31.511, 34.585 and 84.395 kDa, respectively. The majority of these proteins are identified as those involved in stress- and defense-related, disease resistance, as well as gene/protein expression processes. Indeed, these proteins were mostly upregulated during the later storage period suggesting that long-term storage may influence the molecular regulation of COPT sap.
format	Article
author	Marhaini Mostapha, Noorhasmiera Abu Jahar, Kamalrul Azlan Azizan, Sarani Zakaria, Wan Mohd Aizat, Sharifah Nabihah Syed Jaafar,
spellingShingle	Marhaini Mostapha, Noorhasmiera Abu Jahar, Kamalrul Azlan Azizan, Sarani Zakaria, Wan Mohd Aizat, Sharifah Nabihah Syed Jaafar, Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
author_facet	Marhaini Mostapha, Noorhasmiera Abu Jahar, Kamalrul Azlan Azizan, Sarani Zakaria, Wan Mohd Aizat, Sharifah Nabihah Syed Jaafar,
author_sort	Marhaini Mostapha,
title	Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
title_short	Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
title_full	Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
title_fullStr	Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
title_full_unstemmed	Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
title_sort	proteomic analysis of stored core oil palm trunk (copt) sap identifying proteins related to stress, disease resistance and differential gene/protein expression
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12131/ http://journalarticle.ukm.my/12131/ http://journalarticle.ukm.my/12131/1/22%20Marhaini%20Mostapha.pdf
first_indexed	2023-09-18T20:01:55Z
last_indexed	2023-09-18T20:01:55Z
_version_	1777406905901121536

Proteomic analysis of stored core oil palm trunk (COPT) sap identifying proteins related to stress, disease resistance and differential gene/protein expression

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